Pre-synaptic nicotinic receptors evoke endogenous glutamate and aspartate release from hippocampal synaptosomes by way of distinct coupling mechanisms.

British Journal of Pharmacology
Simona ZappettiniMario Marchi

Abstract

The present work aimed to investigate whether and through which mechanisms selective α7 and α4β2 nicotinic receptor (nAChR) agonists stimulate endogenous glutamate (GLU) and aspartate (ASP) release in rat hippocampus. Rat hippocampal synaptosomes were purified on Percoll gradients and superfused in vitro to study endogenous GLU and ASP release. The synaptosomes were superfused with selective α7 and α4β2 nAChR agonists and antagonists. The excitatory amino acid (EAA) content of the samples of superfusate was determined by HPLC after pre-column derivatization and separation on a chromatographic column coupled with fluorimetric detection. Choline (Ch), a selective α7 receptor agonist, elicited a significant release of both GLU and ASP which was blocked by the α7 receptor antagonist methyllycaconitine (MLA), but was unaltered by the α4β2 receptor antagonist dihydro-β-erythroidine (DHβE). The stimulant effect of Ch was strongly reduced in a Ca(2+) -free medium, was not inhibited by Cd(2+) and tetrodotoxin (TTX), but was antagonized by dantrolene, xestospongin C and thapsigargin. 5-Iodo-A-85380 dihydrochloride (5IA85380), a selective α4β2 receptor agonist, elicited EAA release in a DHβE-sensitive, MLA-insensitive fashion. The 5IA8538...Continue Reading

References

Feb 1, 1997·Trends in Neurosciences·S Wonnacott
Jul 22, 1999·Annals of the New York Academy of Sciences·K A RadcliffeJ A Dani
Apr 20, 2000·European Journal of Pharmacology·F ClementiC Gotti
Apr 20, 2000·European Journal of Pharmacology·M AlkondonE X Albuquerque
Nov 4, 2000·Neurochemical Research·L Raiteri, M Raiteri
Jul 4, 2001·Neurochemistry International·M W FleckA M Palmer
Feb 18, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Evelyn K LambeGeorge K Aghajanian
Mar 1, 1960·The Journal of Physiology·D R CURTISJ C WATKINS
Oct 3, 2003·Neurobiology of Learning and Memory·Paul E Gold
Oct 7, 2004·Neuroscience·S E Bradford, J V Nadler
Aug 11, 2007·Biochemical Pharmacology·Bruce E McKayJohn A Dani
Apr 9, 2008·Physiological Reviews·Balázs Lendvai, E Sylvester Vizi

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Citations

Dec 12, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cecilie MorlandVidar Gundersen
Jun 11, 2014·Journal of Chemical Neuroanatomy·Sara FalascaGregor Rainer
Dec 12, 2012·Toxicology and Applied Pharmacology·Kevin D WelchDale R Gardner
Dec 18, 2013·Birth Defects Research. Part C, Embryo Today : Reviews·Benedict T GreenKip E Panter
Jul 28, 2015·Biochemical Pharmacology·Qing Cheng, Jerrel L Yakel
May 29, 2016·Biochimica Et Biophysica Acta·Camila CarignanoGuillermo Spitzmaul
Nov 7, 2019·Journal of Neurochemistry·Trevor W Stone
Feb 2, 2021·Environmental Science and Pollution Research International·Mohsen ShamsiAmeneh Omidi
Jul 16, 2013·Chemical Research in Toxicology·Benedict T GreenStephen T Lee

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