Pre-treatment of central venous catheters with the cathelicidin BMAP-28 enhances the efficacy of antistaphylococcal agents in the treatment of experimental catheter-related infection

Peptides
Oscar CirioniGiorgio Scalise

Abstract

An in vitro antibiotic susceptibility assay for Staphylococcus aureus biofilms developed on 96-well polystyrene tissue culture plates was performed to elucidate the activity of the 27 residues cathelicidin peptide BMAP-28, quinupristin/dalfopristin (Q/D), linezolid, and vancomycin. Efficacy studies were performed in a rat model of staphylococcal CVC infection. Silastic catheters were implanted into the superior cava. Twenty-four hours after implantation the catheters were filled with BMAP-28. Thirty minutes later rats were challenged via the CVC with 1.0x10(6) CFU of S. aureus strain Smith diffuse. Administration of antibiotics into the CVC at a concentration equal to the MBC observed using adherent cells, or at a much higher concentration (1024 microg/mL) began 24 h later. The inhibition activities of all antibiotics against adherent bacteria were at least two-four-fold lower that against freely growing cells. When antibiotics were used in BMAP-28 pre-treated wells, they showed higher activities. The in vivo studies showed that when CVCs were pre-treated with BMAP-28 or with a high dose of antibiotics, biofilm bacterial load was reduced from 10(7) to 10(3) CFU/mL and bacteremia reduced from 10(3) to 10(1) CFU/mL. When CVCs wer...Continue Reading

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Citations

Jul 2, 2011·Biofouling·Cláudia SousaRosário Oliveira
Aug 2, 2007·Expert Opinion on Therapeutic Targets·Neeloffer MookherjeeRobert E W Hancock
Aug 19, 2007·Journal of Pharmaceutical Sciences·Lisa M RossiGeorge Y Wu
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Dec 15, 2018·Microbiology Spectrum·Caroline M GrunenwaldEric P Skaar
Aug 2, 2021·Current Opinion in Microbiology·Ronni A G da SilvaKimberly A Kline

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