Pre-treatment with compound Danshen dripping pills prevents lipid infusion-induced microvascular dysfunction in mice.

Pharmaceutical Biology
Yanda ZhangChun Liang

Abstract

Recent studies have shown compound Danshen dripping pills (CDDP) could improve microcirculation in ischemic/reperfusion injury and other microvascular disorders. The mechanism for CDDP's role in microcirculation is not clear. To explore the protective effects of CDDP on microvascular dysfunction. C57BL/6 male mice (6-8 weeks) were randomized into control, model and CDDP groups (n = 10), which were treated with normal saline or CDDP (105.30 mg/kg), respectively. Then, lipid emulsion and heparin were infused via mice jugular vein to establish systemic microvascular dysfunction model. Coronary flow reserve (CFR) and leukocytes adhesion on microvascular wall were measured. Relative CD11b and CD62L expression levels on neutrophils were measured by flow cytometric analysis. Expression level of forkhead box transcription factor O1 (FOXO1) mRNA was identified by real-time PCR. Lipid infusion significantly attenuated the CFR (1.84 ± 0.14 vs. 2.65 ± 0.02) and increased the number of leukocytes adherent to microvascular wall in cremaster (4067.00 ± 581.20 cells/mm2 vs. 10.67 ± 4.81 cells/mm2). The expression level of CD11b and FOXO1 in neutrophils was also up-regulated by lipid infusion. Pre-treatment with CDDP significantly improved CFR ...Continue Reading

References

Nov 1, 1987·Microvascular Research·S D House, H H Lipowsky
Feb 11, 2009·The Journal of Physiology·S M TurpinM J Watt
Feb 2, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Na ZhaoJing-Yan Han
Oct 15, 2014·Nature Reviews. Cardiology·Paolo G CamiciOrnella Rimoldi
Apr 12, 2017·Chinese Medical Journal·UNKNOWN Writing Group of Recommendations of Expert Panel from Chinese Geriatrics Society on the Clinical Use of Compound Danshen Dri
Sep 21, 2017·Frontiers in Immunology·Guangyu DongDana T Graves
Sep 29, 2017·Circulation Journal : Official Journal of the Japanese Circulation Society·Takanori YasuShinichiro Ueda
Mar 16, 2018·European Heart Journal. Acute Cardiovascular Care·Basmah SafdarAlbert J Sinusas
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenjing LiaoHe Sun
Apr 16, 2019·JACC. Cardiovascular Imaging·Thomas H Schindler, Vasken Dilsizian
Aug 23, 2019·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yanda ZhangZonggui Wu

❮ Previous
Next ❯

Citations

Jul 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ke FengJihong Han

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism
FlowJo
SPSS
Pro Plus
ARRIVE
Image

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.