Preanalytical classical and alternative complement pathway activity loss

Biochemia Medica
Koen O A VercauterenJoris Delanghe

Abstract

Complement functional analyses provide insight into the integrity of the entire complement reaction cascade. These tests are suitable for investigating suspected complement deficiencies. Falsely reduced test outcomes may result from preanalytical instabilities of individual complement components. To generate rationale for this or potential alternative practices, this study aimed to extend the knowledge on the preanalytical stability of widely used tests to screen the complement system. We assessed the influence of time, temperature and EDTA on classical (CH50) and alternative pathway (AP50) functional assay test results. We used nephelometric (C3d) and immunofixation (C3c) techniques to support the investigation of the preanalytical phase of basic complement system activity tests. Quantitative determination of classical and alternative pathway function was performed with a haemolytic activity assay and a C5b-9 neo-epitope ELISA-based assay respectively. Blood of five healthy volunteers was sampled and complement components allowed to degrade under different conditions. CH50 and AP50 remain stable for approximately one week in serum samples incubated on ice. CH50 activity decreased almost twice as fast in EDTA plasma compared to...Continue Reading

References

Aug 11, 1980·FEBS Letters·C L VilliersM G Colomb
Sep 1, 1982·Arthritis and Rheumatism·S TakemuraM Kondo
Nov 26, 1982·Journal of Immunological Methods·M J SinosichJ G Grudzinskas
Feb 21, 2002·Annals of Clinical Biochemistry·Xavier BossuytGuido Vranken
Nov 11, 2003·Clinical and Diagnostic Laboratory Immunology·Michael Kirschfink, Tom E Mollnes
Sep 5, 2007·Molecular Immunology·Tom Eirik MollnesMichael Kirschfink
Aug 20, 2010·Nature Immunology·Daniel RicklinJohn D Lambris
Sep 15, 2010·Cell and Tissue Research·J Vidya Sarma, Peter A Ward
Jun 1, 2011·Molecular Immunology·Lillemor SkattumLennart Truedsson
Jun 28, 2011·Advanced Drug Delivery Reviews·Maria V Carroll, Robert B Sim
Aug 1, 2012·Journal of Clinical Immunology·Ahmed Aziz BousfihaLaurent Abel
Dec 12, 2012·Clinical & Developmental Immunology·Bo Nilsson, Kristina Nilsson Ekdahl
Aug 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Umakhanth Venkatraman GirijaRussell Wallis
Mar 18, 2015·American Journal of Clinical Pathology·Shangbin YangHaifeng M Wu
Oct 7, 2015·American Journal of Hematology·Angela R Shih, Mandakolathur R Murali
Aug 1, 2016·Immunobiology·Zoltán ProhászkaMichael Kirschfink

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Citations

Aug 27, 2021·Frontiers in Immunology·Ashley Frazer-AbelZoltán Prohászka

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Methods Mentioned

BETA
blood draw

Software Mentioned

Prism

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