Preanalytics of urine sediment examination: effect of relative centrifugal force, tube type, volume of sample and supernatant removal

Biochemia Medica
Amalija BunjevacAnita Horvat

Abstract

Laboratories often modify procedures recommended by the European Urinalysis Guidelines for urine sediment analysis. The aim of this study was to compare the recommended protocol with our routine laboratory procedure and to evaluate the possible impact of modifications in the relative centrifugal force, type of tube, method of supernatant aspiration and urine volume on patient's results. Firstly, relative centrifugal force was investigated using 20 pairs of samples examined after centrifugation at 400xg and 1358xg. In phase two, 110 samples were examined, paired as: round bottom vs conical tube (N = 46), decanting vs suction of supernatant (N = 100) and 10 mL vs 5 mL of urine sample (N = 101). The number of erythrocytes, leukocytes and squamous epithelial cells was significantly lower after centrifugation at 400xg (P = 0.001, 0.002 and 0.004, respectively). The number of leukocytes was significantly lower in conical tubes (P = 0.010), after the suction of supernatant (P = 0.045) and in 5 mL urine (P < 0.001). The number of squamous epithelial cells was significantly lower after the suction of supernatant (P < 0.001) and in 5 mL urine (P < 0.001). The number of erythrocytes (P < 0.001), total non-hyaline casts (P < 0.001) and the...Continue Reading

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Citations

Oct 31, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Rui ZhangQingtao Wang
Oct 13, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Dietmar EnkoMarkus Herrmann
May 27, 2021·Journal of Extracellular Vesicles·Uta ErdbrüggerElena S Martens-Uzunova

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Methods Mentioned

BETA
urine collection
urine sediment
blood collection

Software Mentioned

MedCalc

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