Preantral follicular atresia occurs mainly through autophagy, while antral follicles degenerate mostly through apoptosis

Biology of Reproduction
Li MengKatja J Teerds

Abstract

There is a general agreement that granulosa cell apoptosis is the cause of antral follicle attrition. Less clear is whether this pathway is also activated in case of preantral follicle degeneration, as several reports mention that the incidence of granulosa cell apoptosis in preantral follicles is negligible. Our objective is therefore to determine which cell-death pathways are involved in preantral and antral follicular degeneration.Atretic preantal and antral follicles were investigated using immunohistochemistry and laser-capture microdissection followed by quantitative real-time reverse transcription polymerase chain reaction. Microtubule-associated light-chain protein 3 (LC3), sequestosome 1 (SQSTM1/P62), Beclin1, autophagy-related protein 7 (ATG7), and cleaved caspase 3 (cCASP3) were used as markers for autophagy and apoptosis, respectively. P62 immunostaining was far less intense in granulosa cells of atretic compared to healthy preantral follicles, while no difference in LC3 and BECLIN1 immunostaining intensity was observed. This difference in P62 immunostaining was not observed in atretic antral follicles. mRNA levels of LC3 and P62 were not different between healthy and atretic (pre)antral follicles. ATG7 immunostaini...Continue Reading

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Citations

Jun 5, 2020·Journal of Clinical Medicine·Marco ColellaConcetta Ambrosino
Apr 6, 2019·Frontiers in Endocrinology·Jiajie TuWai-Yee Chan
Jul 28, 2020·Journal of Assisted Reproduction and Genetics·Bruce I Rose, Samuel E Brown
Dec 14, 2018·F1000Research·Beverley Vollenhoven, Sarah Hunt
Jul 29, 2020·International Journal of Molecular Sciences·Li MengShouquan Zhang
Dec 7, 2018·Journal of Theoretical Biology·A M PortilloJ A García-Velasco
Apr 4, 2021·International Journal of Molecular Sciences·Li MengSusanne Klaus

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