PMID: 37919Jul 25, 1979

Precipitation of egg white proteins below their isoelectric points by sodium dodecyl sulphate and temperature

Biochimica Et Biophysica Acta
P O Hegg

Abstract

The precipitating of effect of sodium dodecyl sulphate (SDS) on the egg white proteins ovalbumin, conalbumin and lysozyme was studied at 25 degrees C and at different pH values. The proteins precipitated below their respective isolectric points, provided the detergent to protein ratio was appropriate. The pH profile of precipitation was different for the three proteins reflecting net charge differences. The binding of SDS to the proteins was studied with [35S]-labelled SDS and for ovalbumin a ratio of 21--28 SDS molecules/protein molecule, dependent on the concentration of SDS initially used, seem to be required for precipitation at pH 4.5. This number, however, is dependent on pH and increases with an increased positive net charge of the protein. The precipitating effect of SDS was identical for ovalbumin, conalbumin and lysozyme when compared on a gram to gram basis (0.1--0.15 g SDS/g precipitated protein). The precipitated protein was denatured as measured by differential scanning calorimetry, but was also completely redissolved if pH was increased to above the isoelectric point. The precipitating effecto f SDS was also examined at elevated temperatures. The two-phase systems of the proteins induced by SDS at 25 degrees C we...Continue Reading

References

Oct 31, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·T G ParkY Sung Nam
Feb 11, 2015·International Journal of Molecular Sciences·Peter Feist, Amanda B Hummon
May 4, 2005·Biochimica Et Biophysica Acta·Hiroshi ShinoharaY Sugimoto
Jun 21, 2016·Advanced Drug Delivery Reviews·Sam MaherDavid J Brayden
Mar 20, 1997·Biotechnology and Bioengineering·C J CoenH W Blanch
Aug 12, 2003·The Journal of Biological Chemistry·Junji WatanabeThomas M McIntyre

Citations

Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Reynolds, C Tanford

Related Concepts

Conalbumin
Lysozyme Test
Egg White Proteins
Cell Differentiation Process
Lysozyme
Muramidase
Denaturation
Ovalbumin
Calorimetry, Differential Scanning
Irium

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