Precise excision of IS5 from the intergenic region between the fucPIK and the fucAO operons and mutational control of fucPIK operon expression in Escherichia coli.

Journal of Bacteriology
Zhongge ZhangMilton H Saier

Abstract

Excision of transposable genetic elements from host DNA occurs at low frequencies and is usually imprecise. A common insertion sequence element in Escherichia coli, IS5, has been shown to provide various benefits to its host by inserting into specific sites. Precise excision of this element had not previously been demonstrated. Using a unique system, the fucose (fuc) regulon, in which IS5 insertion and excision result in two distinct selectable phenotypes, we have demonstrated that IS5 can precisely excise from its insertion site, restoring the wild-type phenotype. In addition to precise excision, several "suppressor" insertion, deletion, and point mutations restore the wild-type Fuc(+) phenotype to various degrees without IS5 excision. The possible bases for these observations are discussed.

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·E LjungquistA I Bukhari
Jan 1, 1978·Journal of Bacteriology·D R McCallaM H Green
Jan 1, 1975·Molecular & General Genetics : MGG·H J Reif, H Saedler
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Schnetz, B Rak
May 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·S SatolaC P Moran
Sep 5, 1986·Journal of Molecular Biology·A Ahmed
Jan 1, 1974·Molecular & General Genetics : MGG·H SaedlerN Davidson
Nov 18, 1983·Science·T A WeinertN D Grindley
Mar 1, 1984·Journal of Bacteriology·T ChakrabartiE C Lin
Sep 5, 1997·Science·F R BlattnerY Shao
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 18, 2009·Molecular Microbiology·Zhongge Zhang, Milton H Saier
Oct 17, 2009·PLoS Genetics·Zhongge Zhang, Milton H Saier

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Citations

Mar 15, 2011·Journal of Molecular Evolution·Joël GafféDominique Schneider
Mar 11, 2011·The ISME Journal·Xiaoxue Wang, Thomas K Wood
Jan 18, 2012·Journal of Molecular Microbiology and Biotechnology·Milton H Saier
Jan 18, 2012·Journal of Molecular Microbiology and Biotechnology·Zhongge Zhang, Milton H Saier
Oct 17, 2009·PLoS Genetics·Zhongge Zhang, Milton H Saier
Nov 10, 2010·PLoS Computational Biology·Dipak BaruaJennifer L Reed
Jan 20, 2017·Microbiology·Zhongge ZhangMilton H Saier
Nov 10, 2012·ACS Chemical Biology·Anubrata GhosalPeter E Nielsen
Aug 17, 2020·Metabolic Engineering·Anke R GoormansWim K Soetaert

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