May 20, 2015

Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9

Methods in Molecular Biology
Scott J GratzKate M O'Connor-Giles

Abstract

The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.

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Mentioned in this Paper

Genome
CRISPR-Cas Systems
Base Excision Repair
Gene Deletion
Drosophila
Injection Procedure
Homologous Recombination
Embryonic Structures, Nonmammalian
Streptococcus pyogenes
Repeat-Associated Mysterious Proteins

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