Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Zhi-Jie LiangHong-Mian Li

Abstract

The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment. Third-passage NFSCs were isolated and cultured using a mechanical emulsification method and their surface CD markers were analyzed by flow cytometry. The adipogenic and osteogenic nature and chondrogenic differentiation capacity of NFSCs were determined using Oil Red O staining, alizarin red staining, and Alcian blue staining, respectively. Paracrine function of NFSCs was evaluated by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7, 14, and 28 days after establishing the culture. Then, the effects of PRF on NFSC proliferation were assessed in vitro. Finally, we compared the outcome in 103 patients with facial skin aging who underwent both nanofat and intradermal PRF injection (treatment group) and 128 patients who underwent hy...Continue Reading

Citations

Mar 1, 2019·The Journal of Craniofacial Surgery·Jack E BrookerKacey G Marra
Apr 20, 2019·Aesthetic Surgery Journal·Yizuo CaiWei Li
Aug 4, 2019·Journal of Periodontology·Jila NasirzadeReinhard Gruber
Feb 26, 2020·Plastic and Reconstructive Surgery. Global Open·Sophie MenkesLuigi Polla
Oct 9, 2019·Plastic and Reconstructive Surgery. Global Open·Steven R Cohen, Hayley Womack
May 3, 2020·Aesthetic Surgery Journal·Mohammad GhiaslooPatrick Tonnard
Sep 19, 2019·Oxidative Medicine and Cellular Longevity·Mingwu DengWenjie Zhang
Nov 7, 2019·Plastic and Reconstructive Surgery·Joris A van DongenBerend van der Lei
Jun 27, 2020·Plastic and Reconstructive Surgery·Joris A van DongenBerend van der Lei
Jun 27, 2020·Plastic and Reconstructive Surgery·Denis Souto Valente, Rafaela Koehler Zanella
Dec 20, 2019·Annals of Plastic Surgery·Ruobing AnWeigang Cao
Nov 30, 2019·Facial Plastic Surgery Clinics of North America·Aunna PourangKian Karimi
Jun 7, 2021·International Journal of Oral and Maxillofacial Surgery·S I MouradR R El-Zehary
Oct 14, 2019·ACS Biomaterials Science & Engineering·Hui ChiJinglong Yan

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