Precise repair of mPing excision sites is facilitated by target site duplication derived microhomology

Mobile DNA
David M GilbertC Nathan Hancock

Abstract

A key difference between the Tourist and Stowaway families of miniature inverted repeat transposable elements (MITEs) is the manner in which their excision alters the genome. Upon excision, Stowaway-like MITEs and the associated Mariner elements usually leave behind a small duplication and short sequences from the end of the element. These small insertions or deletions known as "footprints" can potentially disrupt coding or regulatory sequences. In contrast, Tourist-like MITEs and the associated PIF/Pong/Harbinger elements generally excise precisely, returning the genome to its original state. The purpose of this study was to determine the mechanisms underlying these excision differences, including the role of the host DNA repair mechanisms. The transposition of the Tourist-like element, mPing, and the Stowaway-like element, 14T32, were evaluated using yeast transposition assays. Assays performed in yeast strains lacking non-homologous end joining (NHEJ) enzymes indicated that the excision sites of both elements were primarily repaired by NHEJ. Altering the target site duplication (TSD) sequences that flank these elements reduced the transposition frequency. Using yeast strains with the ability to repair the excision site by ho...Continue Reading

References

Feb 11, 1991·Nucleic Acids Research·A DoseffV Sundaresan
May 1, 1989·Genes & Development·M C MullinsG M Rubin
Dec 1, 1995·Current Opinion in Genetics & Development·S R WesslerS E White
Feb 5, 1998·The Plant Journal : for Cell and Molecular Biology·T A RinehartC F Weil
Mar 18, 1999·Nature Genetics·G GiaeverR W Davis
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·A M CasaS R Wessler
May 24, 2001·Molecular Genetics and Genomics : MGG·C Augé-GouillouY Bigot
Aug 28, 2001·Nucleic Acids Research·L ZhangD J Finnegan
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·X ZhangS R Wessler
May 4, 2002·Nature Reviews. Genetics·Cédric FeschotteSusan R Wessler
Jun 21, 2002·Methods in Enzymology·R Daniel Gietz, Robin A Woods
Jan 10, 2003·Nature·Ning JiangSusan R Wessler
Jul 29, 2004·DNA Repair·Yael Aylon, Martin Kupiec
Nov 16, 2005·Annual Review of Genetics·James M DaleyThomas E Wilson
Oct 17, 2006·The Plant Cell·Guojun YangSusan R Wessler
Nov 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ken NaitoSusan R Wessler
Feb 23, 2007·Yeast·Ivan SadowskiJennifer Parent
Jun 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Guojun YangSusan R Wessler
Nov 7, 2007·Nature Reviews. Genetics·Thomas WickerAlan H Schulman
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yao-Wu Yuan, Susan R Wessler

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Citations

May 18, 2016·Chemical Reviews·Alison B Hickman, Fred Dyda
Jan 29, 2021·Plant Direct·Alexander JohnsonC Nathan Hancock
Oct 15, 2021·PLoS Genetics·Michelle C StitzerJeffrey Ross-Ibarra

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Methods Mentioned

BETA
PCR
restriction digest
electrophoresis

Software Mentioned

MU
hAT
CACTA
MuDR
mPing

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