Precise small volume sample handling for capillary electrophoresis

Electrophoresis
Mona MozafariSami El Deeb

Abstract

CE is one of the most important analytical techniques. Although the injected sample volume in CE is only in the nanoliter range, most commercial CE instruments need approximately 50 μL of the sample in the injection vial to perform the analysis. Hence, in order to fully profit from the low injection volumes, smaller vial volumes are required. Thus experiments were performed using silicone oil, which has higher density than water (1.09 g/mL) to replace sample dead volume in the vial. The results were compared to those performed without using the silicone oil in the sample vial. As an example five standard proteins namely beta-lactoglobulin, BSA, HSA, myoglobin, and ovalbumin, and one of the coagulation cascade involved proteins called vitonectin were investigated using CE. Mobility ratios and peak areas were compared. However, no significant changes were observed (RSDs% for mobility ratios and peak areas were better than 0.9 and 5.8%, respectively). Afterwards, an affinity CE method was used to investigate the interactions of two proteins, namely HSA and vitronectin, with three ligands namely enoxaparin sodium, unfractionated heparin, and pentosan polysulfate sodium. Mobility shift precision results showed that the employment of...Continue Reading

References

Jul 26, 2002·Analytical Chemistry·Walter R Vandaveer, Ingrid Fritsch
Jan 19, 2010·Journal of Pharmaceutical and Biomedical Analysis·Deia El-HadyHermann Wätzig
Dec 24, 2014·Bioanalysis·Hassan M AlbishriHermann Wätzig
Feb 2, 2015·Journal of Pharmaceutical and Biomedical Analysis·Hassan A AlhazmiHermann Wätzig

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