Precisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing

BMC Genomics
Genqiao LiLiuling Yan

Abstract

One of the reasons hard red winter wheat cultivar 'Duster' (PI 644016) is widely grown in the southern Great Plains is that it confers a consistently high level of resistance to biotype GP of Hessian fly (Hf). However, little is known about the genetic mechanism underlying Hf resistance in Duster. This study aimed to unravel complex structures of the Hf region on chromosome 1AS in wheat by using genotyping-by-sequencing (GBS) markers and single nucleotide polymorphism (SNP) markers. Doubled haploid (DH) lines generated from a cross between two winter wheat cultivars, 'Duster' and 'Billings' , were used to identify genes in Duster responsible for effective and consistent resistance to Hf. Segregation in reaction of the 282 DH lines to Hf biotype GP fit a one-gene model. The DH population was genotyped using 2,358 markers developed using the GBS approach. A major QTL, explaining 88% of the total phenotypic variation, was mapped to a chromosome region that spanned 178 cM and contained 205 GBS markers plus 1 SSR marker and 1 gene marker, with 0.86 cM per marker in genetic distance. The analyses of GBS marker sequences and further mapping of SSR and gene markers enabled location of the QTL-containing linkage group on the short arm o...Continue Reading

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Citations

Nov 26, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Meiyan LiuLiuling Yan
Nov 7, 2015·Journal of Insect Science·Brandon J SchemerhornRichard H Shukle
Jun 19, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chor-Tee TanShuyu Liu
Sep 25, 2017·Molecular Breeding : New Strategies in Plant Improvement·Jiayin SongCharles Chen
May 22, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lanfei ZhaoGuihua Bai
Jul 21, 2017·The Plant Genome·Yaopeng ZhouJosé Costa
Mar 4, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bingzhi JiangHai Nian
Apr 14, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Tilin FangLiuling Yan
Jul 4, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Fuan NiuGuihua Bai
Feb 11, 2021·Plants·Pasqualina ColasuonnoJose Miguel Soriano

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Datasets Mentioned

BETA
SRP051982
KF035081

Methods Mentioned

BETA
chips
genotyping
PCR

Software Mentioned

TCAP
MapQTL
Interval
JoinMap

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