Precision and accuracy of a transportable dual-energy X-ray absorptiometry unit for bone mineral measurements in guinea pigs

Calcified Tissue International
Christian FinkVirginia B Kraus

Abstract

Dual energy X-ray absorptiometry (DXA) is a valuable tool for measuring bone mineral content (BMC) and bone mineral density (BMD) in small-animal research. The present study was devised to establish guidelines and to define sites for bone mineral measurements in guinea pigs and to evaluate the accuracy of a new transportable research DXA unit. Repeated scans were performed on 30 guinea pig hindlimbs (in situ) as well as the isolated bones from these limbs (ex situ). Nine exactly specified regions of interest (ROIs) were analyzed twice for BMC and BMD by three different observers. Additionally, the BMC of whole bones and bone segments as measured by DXA was correlated to ash weights of bone in a subset of five animals to determine the accuracy of the DXA measurements. On ex situ scans, intra-observer variability for BMD ranged from 0.09% to 2.33% and inter-observer variability from 0.23% to 5.86% depending on the site studied, with smaller ROIs exhibiting more variability. Coefficients of variance (CV) for BMC measurements were slightly higher than for BMD. However, BMC offered a better correlation between in situ and ex situ values than BMD. On in situ scans, observer variability for BMD and BMC for comparable sites was higher ...Continue Reading

Citations

Jan 13, 2004·Journal of Clinical Densitometry : the Official Journal of the International Society for Clinical Densitometry·Jason A HortonTimothy A Damron
Jun 10, 2004·Arthritis and Rheumatism·Virginia B KrausAmy G Clark
Feb 6, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N BinkleyR J Colman
Dec 5, 2009·The Journal of Veterinary Medical Science·Masashi TSUJIOShin Tanaka

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