Precision breeding for RNAi suppression of a major 4-coumarate:coenzyme A ligase gene improves cell wall saccharification from field grown sugarcane

Plant Molecular Biology
Je Hyeong JungFredy Altpeter


Sugarcane (Saccharum spp. hybrids) is a major feedstock for commercial bioethanol production. The recent integration of conversion technologies that utilize lignocellulosic sugarcane residues as well as sucrose from stem internodes has elevated bioethanol yields. RNAi suppression of lignin biosynthetic enzymes is a successful strategy to improve the saccharification of lignocellulosic biomass. 4-coumarate:coenzyme A ligase (4CL) is a key enzyme in the biosynthesis of phenylpropanoid metabolites, such as lignin and flavonoids. Identifying a major 4CL involved in lignin biosynthesis among multiple isoforms with functional divergence is key to manipulate lignin biosynthesis. In this study, two full length 4CL genes (Sh4CL1 and Sh4CL2) were isolated and characterized in sugarcane. Phylogenetic, expression and RNA interference (RNAi) analysis confirmed that Sh4CL1 is a major lignin biosynthetic gene. An intragenic precision breeding strategy may facilitate the regulatory approval of the genetically improved events and was used for RNAi suppression of Sh4CL1. Both, the RNAi inducing cassette and the expression cassette for the mutated ALS selection marker consisted entirely of DNA sequences from sugarcane or the sexually compatible s...Continue Reading


Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·W J HuV L Chiang
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·J EhltingE Kombrink
Nov 24, 1999·Journal of Agricultural and Food Chemistry·R D HatfieldK Brei
Oct 25, 2000·Trends in Plant Science·E Pichersky, D R Gang
Mar 1, 2003·Nature Biotechnology·Kaare M Nielsen
Sep 1, 2004·Trends in Plant Science·Caius M Rommens
Jun 19, 2007·Nature Biotechnology·Fang Chen, Richard A Dixon
Aug 19, 2007·Trends in Plant Science·Caius M RommensWilliam R Belknap
Sep 14, 2007·Transgenic Research·J Troy WeeksCaius M Rommens
May 20, 2008·Trends in Plant Science·Henk J Schouten, Evert Jacobsen
Oct 31, 2008·Plant Physiology·Armin WagnerJohn Ralph
Dec 26, 2009·Molecular Plant·Thomas Vogt
Dec 31, 2009·Plant & Cell Physiology·Shingo NagayaKo Kato
Jun 25, 2010·Plant Biotechnology Journal·Katherine M HanSteven H Strauss
Aug 14, 2010·Science·Chris SomervilleStephen P Long
Sep 3, 2010·Annual Review of Genetics·Nicholas D Bonawitz, Clint Chapple
Feb 12, 2011·Plant, Cell & Environment·Steven L VoelkerSteven H Strauss
Jun 15, 2011·Journal of Biotechnology·Thalia VanblaereCesare Gessler
Oct 1, 2011·Plant Biotechnology Journal·Inger B HolmePreben B Holm
Oct 11, 2011·Current Opinion in Biotechnology·Maximiller Dal-BiancoGlaucia Mendes Souza
Feb 9, 2012·The Plant Journal : for Cell and Molecular Biology·Ana SaballosWilfred Vermerris
Feb 21, 2013·Plant Biotechnology Journal·Inger Bæksted HolmePreben Bach Holm
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Apr 23, 2014·Plant Biotechnology Journal·Holly L BaxterC Neal Stewart
Jul 24, 2014·Plant Biotechnology Journal·Charleson R PoovaiahCharles N Stewart
Dec 23, 2014·Current Opinion in Biotechnology·Wilfred Vermerris, Alejandra Abril
Jun 11, 2015·Plant Biotechnology Journal·Janice ZaleFredy Altpeter

❮ Previous
Next ❯


May 14, 2019·Biotechnology for Biofuels·Wagner Rodrigo de SouzaHugo Bruno Correa Molinari
Mar 4, 2020·Molecular Genetics and Genomics : MGG·Douglas Jardim-MessederGilberto Sachetto-Martins
Apr 26, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhiqiang WangYaxi Liu
Jan 7, 2021·Functional & Integrative Genomics·Douglas Jardim-MessederGilberto Sachetto-Martins
Mar 9, 2021·Frontiers in Plant Science·Maria Juliana Calderan-RodriguesCamila Caldana
Mar 20, 2021·Plant Molecular Biology·K Hodgson-KratkyR J Henry
Mar 29, 2021·International Journal of Biological Macromolecules·Santosh G LavhaleAshok P Giri
Aug 25, 2020·New Biotechnology·Ewa WoźniakTomasz Twardowski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.