Precision cell delivery in biphasic polymer systems enhances growth of keratinocytes in culture and promotes their attachment on acellular dermal matrices

Journal of Tissue Engineering and Regenerative Medicine
Rishima AgarwalJohn P Frampton

Abstract

Current approaches for precision deposition of cells are not optimized for moist environments or for substrates with complex surface topographic features, for example, the surface of dermal matrices and other biomaterials. To overcome these challenges, an approach is presented that utilizes cell confinement in phase-separating polymer solutions of polyethylene glycol and dextran to precisely deliver keratinocytes in well-defined colonies. Using this approach, keratinocyte colonies are produced with superior viability, proliferative capacity, and barrier formation compared with the same number of cells dispersedly seeded across substrate surfaces. It is further demonstrated that keratinocytes delivered in colonies to the surface of acellular dermal matrices form an intact epidermal basal layer more rapidly and more completely than cells delivered by conventional dispersed seeding. These findings demonstrate that delivery of keratinocytes in phase-separating polymer solutions holds potential for enhancing growth of keratinocytes in culture and production of functional skin equivalents.

References

Dec 1, 1994·Burns : Journal of the International Society for Burn Injuries·J M StillE J Law
Jun 13, 1998·Burns : Journal of the International Society for Burn Injuries·R J WalterM Hanumadass
Feb 9, 2000·Archives of Dermatological Research·P B Sugerman, M Bigby
Feb 24, 2001·The Journal of Burn Care & Rehabilitation·F A NavarroD P Orgill
Apr 25, 2002·Nature Reviews. Genetics·Elaine Fuchs, Srikala Raghavan
Jan 26, 2007·Journal of the Royal Society, Interface·Anthony D Metcalfe, Mark W J Ferguson
Feb 23, 2007·Nature·Sheila MacNeil
Nov 26, 2010·Hernia : the Journal of Hernias and Abdominal Wall Surgery·J A FalerisD C Greenspan
Jul 6, 2014·Burns : Journal of the International Society for Burn Injuries·Celine AuxenfansFabienne Braye
Sep 12, 2015·ACS Applied Materials & Interfaces·Ehsan AtefiHossein Tavana
May 12, 2016·Trends in Biotechnology·Wei Long NgMay Win Naing
Apr 8, 2017·International Journal of Molecular Sciences·Komal VigShree R Singh
Jul 3, 2017·Advanced Drug Delivery Reviews·Britt Ter HorstLiam M Grover
Sep 21, 2017·Scientific Reports·Jos P H SmitsEllen H van den Bogaard
Nov 17, 2017·Nature·Tobias HirschMichele De Luca
Dec 28, 2017·Advanced Healthcare Materials·Alyne G TeixeiraJohn P Frampton
Feb 7, 2018·Burns and Trauma·Peng HeXiaolu Li

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Citations

Mar 8, 2020·Biophysics Reviews·Kanta TsumotoKenichi Yoshikawa
Mar 2, 2021·ACS Applied Materials & Interfaces·Chris J PeacockJohn P Frampton
Jan 29, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Charles D Crowe, Christine D Keating

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