Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics.

NPJ Precision Oncology
Hoyoon LeeSehyun Shin

Abstract

Cell-free DNA (cfDNA) has been implicated as an important biomarker in cancer management. Thus, efficient techniques for cfDNA extraction are necessary for precision medicine. We developed a centrifugation-free cfDNA extraction microfluidic chip capable of extracting cfDNA from plasma samples through microfluidic circuits within 15 min under vacuum pressure using an immiscible solvent. The microfluidic chip had excellent performance that was comparable to the most widely used commercial product (QIAamp kit) in terms of extraction efficiency, purity, and quality of DNA samples. The microfluidic chip was validated for the continuous monitoring of HER-2 type breast cancer and was able to successfully detect a point mutation in phosphatidylinositol-4,5-bisphosphate 3-kinase (PIK3CA) during severe liver metastasis. The chip effectively eliminates the repetitive centrifugation processes and dramatically shortened the sample preparation time. The proposed platform could facilitate the development of a sample-to-answer system for use in liquid biopsy of cancers.

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Citations

Mar 4, 2020·Journal of Clinical Medicine·Jinhyun KimSehyun Shin
Jun 24, 2020·Biomedical Microdevices·Athina S KastaniaEvangelos Gogolides

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Methods Mentioned

BETA
biopsy
solid phase
nucleic acid extraction
chips
chip
PCR
biopsies
Assay

Software Mentioned

PIBEX
QIAamp

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