PMID: 9167895Apr 1, 1997Paper

Precision of the magnesium determination in mononuclear blood cells and erythrocytes

Clinical Biochemistry
H J HuijgenG T Sanders

Abstract

Establishing the analytical variation and reproducibility of the intracellular magnesium (Mg) assay in mononuclear blood cells (MBC) and erythrocytes (RBC). We assessed the analytical variation of the several determination steps, and the reproducibility for the complete intracellular Mg-assay (combination of preanalytical, analytical, and biological variation). The influence of platelets was determined by comparing Mg concentrations obtained from heparinized blood and defibrinated blood. Coefficients of variation of the several determination steps used in the MBC- and RBC-assay were < or = 5.4%. The overall analytical variation was 5.0-6.8%, and reproducibility of the complete Mg-assay 11.6-14.0%. Mg measurements in MBC (expressed as fmol/cell) obtained from heparinized blood showed significantly higher values than those obtained from defibrinated blood. This is the first study to describe in detail reproducibility data for the individual steps in the overall procedure to measure intracellular magnesium. It is shown that results obtained in daily practice should be interpreted with care. Moreover, the removal of platelets is essential in the determination of Mg in MBC.

References

Aug 14, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A ReinhartN A Desbiens
Sep 1, 1993·Annals of Clinical Biochemistry·P M KempW F Sampson
Jan 1, 1993·Annals of Clinical Biochemistry·B J MartinG S Fell

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Citations

Mar 27, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·R SandersG T Sanders
Nov 9, 2000·American Journal of Clinical Pathology·H J HuijgenG T Sanders

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