Preclinical evaluation of lime juice as a topical microbicide candidate

Retrovirology
Patricia S FletcherRobin J Shattock

Abstract

The continued growth of the global HIV epidemic highlights the urgent need to develop novel prevention strategies to reduce HIV transmission. The development of topical microbicides is likely to take a number of years before such a product would be widely available. This has resulted in a call for the rapid introduction of simpler vaginal intervention strategies in the interim period. One suggested practice would be vaginal douching with natural products including lime or lemon juice. Here we present a comprehensive preclinical evaluation of lime juice (LiJ) as a potential intervention strategy against HIV. Pre-treatment of HIV with LiJ demonstrated direct virucidal activity, with 10% juice inactivating the virus within 5 minutes. However, this activity was significantly reduced in the presence of seminal plasma, where inactivation required maintaining a 1:1 mixture of neat LiJ and seminal plasma for more than 5 minutes. Additionally, LiJ demonstrated both time and dose-dependent toxicity towards cervicovaginal epithelium, where exposure to 50% juice caused 75-90% toxicity within 5 minutes increasing to 95% by 30 minutes. Cervicovaginal epithelial cell monolayers were more susceptible to the effects of LiJ with 8.8% juice causi...Continue Reading

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Apr 1, 2011·Drug Delivery and Translational Research·Meredith R ClarkDavid R Friend
Oct 13, 2010·Toxicology·Seyoum AyehunieMitchell Klausner
Nov 13, 2009·Social Science & Medicine·Adriane Martin HilberUNKNOWN WHO GSVP Study Group
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Methods Mentioned

BETA
lavages
hysterectomy

Software Mentioned

GraphPad
Graphpad PRISM
ACIDFORM

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