Preclinical in vivo evaluation of NPe6-mediated photodynamic therapy on normal vasculature.

Lasers in Surgery and Medicine
Wesley J MoyBernard Choi

Abstract

Current treatments of port-wine stain birthmarks typically involve use of a pulsed dye laser (PDL) combined with cooling of the skin. Currently, PDL therapy protocols result in varied success, as some patients experience complete blanching, while others do not. Over the past decade, we have studied the use of photodynamic therapy (PDT) as either a replacement or adjuvant treatment option to photocoagulate both small and large vasculature. The objective of the current study was to evaluate a PDT protocol that involves use of an alternate intravascular photosensitizer mono-L-aspartylchlorin-e6 (NPe6) activated by an array of low-cost light emitting diodes. To monitor the microvasculature, a dorsal window chamber model was installed on 22 adult male mice. The light source consisted of a custom-built LED array that emitted 10 W at a center wavelength of 664 nm (FWHM = 20 nm). The light source was positioned at a fixed distance from the window chamber to achieve a fixed irradiance of 127 mW/cm(2). A retroorbital injection of NPe6 (5 mg/kg) was performed to deliver the drug into the bloodstream. Laser irradiation was initiated immediately after injection. To monitor blood-flow dynamics in response to PDT, we used laser speckle imagin...Continue Reading

References

May 6, 1999·Lasers in Surgery and Medicine·J K BartonA J Welch
Apr 20, 2000·Journal of Endourology·K F ChanA J Welch
Jul 28, 2007·The Journal of Investigative Dermatology·Bernard ChoiJustin Lotfi
Aug 19, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jitsuo UsudaHarubumi Kato
Sep 18, 2007·Lasers in Surgery and Medicine·Anne M ChapasRoy G Geronemus
May 10, 2008·Journal of Biomedical Optics·Gracie VargasAshley J Welch
May 14, 2008·Neoplasia : an International Journal for Oncology Research·Soumya Mitra, Thomas H Foster
Jun 11, 2008·Optics Express·Julio C Ramirez-San-JuanBernard Choi
Oct 28, 2008·Lasers in Surgery and Medicine·Jennifer ChannualKristen M Kelly
Nov 26, 2009·Photodiagnosis and Photodynamic Therapy·Joshua A TournasKristen M Kelly

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Citations

Aug 1, 2015·Lasers in Surgery and Medicine·Wesley J MoyBernard Choi
Mar 26, 2016·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Bernard ChoiJ Stuart Nelson
Dec 25, 2015·Optics Express·Hakan ErkolGultekin Gulsen
Jul 19, 2014·The Journal of Investigative Dermatology·Kristen M KellyBernard Choi
Jan 8, 2015·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Takehiro KimuraKeiichi Fukuda
Mar 31, 2015·Burns : Journal of the International Society for Burn Injuries·Christian CrouzetBernard Choi
May 11, 2017·Lasers in Surgery and Medicine·Wesley J MoyBernard Choi
Mar 17, 2018·Journal of Biomedical Optics·Ben LertsakdadetBernard Choi
Oct 28, 2019·Biomedical Optics Express·Ben LertsakdadetBernard Choi
Oct 20, 2018·Biomedical Optics Express·Michael GhijsenBruce J Tromberg

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