Preclinical study of a cost-effective photodynamic therapy protocol for treating oral candidoses

Lasers in Medical Science
Nathalia Ramos da SilvaRaphael Freitas de Souza

Abstract

Photodynamic therapy (PDT) is a promising treatment for oral candidoses. Its use as an alternative to antifungals prevents several adverse effects, including microbial resistance. However, most PDT protocols do not employ devices and consumables commonly available in dental practice, thus influencing treatment affordability. This study aimed to determine the efficacy of a PDT method based on light curing units' blue LEDs combined to a plaque-disclosing composition (5% erythrosine) against C. albicans in culture and in a murine model of oral candidosis. Standard and resistant fungal strains were tested in vitro in planktonic and biofilm forms. PDT (pre-irradiation time periods: 30 and 60 s; irradiation time: 3 min) was compared to control conditions without light and/or erythrosine. Mice with induced oral candidosis (n = 40) randomly received PDT or similar control conditions with subsequent C. albicans count. These mice underwent histological analysis, as well as 12 healthy mice submitted to experimental treatments. PDT completely inactivated C. albicans planktonic cells and biofilm. Control conditions presented minor differences (ANOVA, p < 0.05), with mean values ranging from 5.2 to 6.8 log10 (UFC/mL). Infected mice presented...Continue Reading

References

Jan 1, 1981·Mutation Research·R B Haveland-SmithB A Bridges
Jan 1, 1994·Annals of Surgical Oncology·H I PassJ W Cole
Oct 26, 1999·Drug Discovery Today·W M Sharman van Lier JE
Feb 28, 2002·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·M C TeichertM A Biel
Aug 20, 2002·Postgraduate Medical Journal·A Akpan, R Morgan
Jun 27, 2003·Microbiology and Immunology·Natsuko TakakuraShigeru Abe
Sep 17, 2003·Oral Diseases·C Scully
May 3, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Swati DeAgnishwar Girigoswami
Feb 9, 2006·The Journal of Antimicrobial Chemotherapy·Simon WoodColin Robinson
Jun 1, 2006·The Journal of Antimicrobial Chemotherapy·Daniel MetcalfSimon Wood
Sep 20, 2006·Journal of Endodontics·Nikolaos S SoukosPhilip P Stashenko
May 2, 2009·Lasers in Medical Science·Juliana Campos JunqueiraAntonio Olavo Cardoso Jorge
Jun 24, 2009·Gerodontology·Helena de Freitas Oliveira ParanhosKarina Matthes de Freitas Pontes
Sep 26, 2009·British Dental Journal·L Coulthwaite, J Verran
Nov 3, 2009·Periodontology 2000·Aristeo A TakasakiYuichi Izumi
Jan 12, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Ewerton Garcia de Oliveira MimaVanderlei Salvador Bagnato
Jan 16, 2010·Lasers in Surgery and Medicine·Tianhong DaiMichael R Hamblin
Feb 16, 2010·Odontology·Masakazu NiimiRichard D Cannon
Mar 17, 2010·Lasers in Medical Science·Rodnei D RossoniAntonio Olavo C Jorge
Dec 24, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Joyce da Silva MartinsAntonio Olavo Cardoso Jorge
Dec 25, 2010·Brazilian Oral Research·Anna Carolina Borges Pereira A CostaAntonio Olavo Cardoso Jorge
Jun 15, 2011·Mycoses·Anna Carolina Borges Pereira CostaAntonio Olavo Cardoso Jorge
Jun 28, 2011·Archives of Oral Biology·Anna Carolina Borges Pereira CostaAntonio Olavo Cardoso Jorge
Jun 26, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Anna Carolina Borges Pereira CostaAntonio Olavo Cardoso Jorge
Sep 1, 2012·Medical Mycology·Lívia Nordi DovigoAna Cláudia Pavarina
Feb 21, 2013·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·P Anitha Krishnan
May 1, 2004·Photodiagnosis and Photodynamic Therapy·Ron R AllisonClaudio H Sibata
Jul 15, 2015·Journal of Photochemistry and Photobiology. B, Biology·Ayaka YoshidaFumihiko Yoshino
Sep 24, 2015·Journal of Photochemistry and Photobiology. B, Biology·Cristiane Aparecida PereiraAntonio Olavo Cardoso Jorge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.