Preconditioning human cardiomyocytes and endothelial cells

The Journal of Thoracic and Cardiovascular Surgery
T ShiraiD Mickle

Abstract

The effects of simulated "ischemia" and "reperfusion" were evaluated in cell cultures of human ventricular cardiomyocytes and human saphenous vein endothelial cells. Myocyte and endothelial cell cultures were exposed to a low volume (1.5 ml) of either hypoxic (oxygen tension = 16 mm Hg) or anoxic (oxygen tension = 0 mm Hg) phosphate-buffered saline solution for 90 minutes ("ischemia") followed by 30 minutes of simulated "reperfusion." Cell injury was evaluated by trypan blue exclusion. Next, the effects of a preconditioning stimulus were evaluated by a brief (10 minute) exposure to hypoxic or anoxic ischemia and 10 minutes of reperfusion before prolonged (90 minutes) anoxic ischemia. Finally, the effects of anoxic preconditioning on intracellular lactate accumulation and extracellular lactate and acid release were assessed. "Ischemia" and "reperfusion" resulted in greater injury to endothelial cells than to cardiomyocytes. In both cell types, anoxic ischemia resulted in greater injury than hypoxic ischemia. Preconditioning reduced cell injury in myocytes but not in endothelial cells. Endothelial cells produced more lactate than cardiomyocytes under normoxic conditions. Ischemia increased lactate accumulation and release in card...Continue Reading

References

Jun 1, 1992·Journal of Molecular and Cellular Cardiology·R K LiD Mickle
Jun 1, 1992·Circulation·M OvizeR A Kloner
Oct 1, 1991·Circulation Research·P AnversaJ M Capasso
Feb 1, 1989·Journal of Molecular and Cellular Cardiology·R SpahrH M Piper
Jul 1, 1986·Journal of Molecular and Cellular Cardiology·S L Jacobson, H M Piper
Sep 1, 1995·The Annals of Thoracic Surgery·J F Seccombe, H V Schaff
Oct 1, 1994·Journal of the American College of Cardiology·R A Kloner, D Yellon

❮ Previous
Next ❯

Citations

Aug 11, 2000·Transplantation Proceedings·M ShimadaJ G Coles
Dec 20, 2000·Transplantation Proceedings·M ShimadaJ G Coles
Dec 3, 2013·Canadian Journal of Physiology and Pharmacology·Sreejit ParameswaranRajendra K Sharma
Aug 29, 2012·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Paul MagillKevin J Mulhall
Apr 24, 2012·Journal of Molecular and Cellular Cardiology·Robert M Bell, Derek M Yellon
Mar 18, 2000·The Journal of Surgical Research·T H Pohlman, J M Harlan
Jun 20, 2008·Journal of Molecular and Cellular Cardiology·Husnain Kh Haider, Muhammad Ashraf
May 16, 2000·Circulation Research·M S Marber
Apr 28, 2005·Journal of Cardiac Surgery·Mustafa BuyukatesHalim Soncul
Jun 8, 2019·European Heart Journal. Acute Cardiovascular Care·Xavier RosselloBorja Ibanez
Nov 27, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Naoto FukunagaVivek Rao
Oct 29, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A T SaurinM S Marber
Feb 9, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Noritsugu ShionoSubodh Verma
Sep 25, 2003·Physiological Reviews·Derek M Yellon, James M Downey
Nov 20, 2016·International Journal of Pediatric Otorhinolaryngology·Jia Xian LawBt Hj Idrus Ruszymah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Rutger-Jan SwijnenburgRobert C Robbins
American Journal of Physiology. Heart and Circulatory Physiology
Marcus D DarrabieDanny O Jacobs
Basic & Clinical Pharmacology & Toxicology
Jeppe Grøndahl Rasmussen, Ulf Simonsen
© 2021 Meta ULC. All rights reserved