Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress

Biomaterials
Jun WatanabeHiroshi Egusa

Abstract

Oxidative stress on transplanted bone marrow-derived mesenchymal stem cells (BMSCs) during acute inflammation is a critical issue in cell therapies. N-acetyl-L cysteine (NAC) promotes the production of a cellular antioxidant molecule, glutathione (GSH). The aim of this study was to investigate the effects of pre-treatment with NAC on the apoptosis resistance and bone regeneration capability of BMSCs. Rat femur-derived BMSCs were treated in growth medium with or without 5 mM NAC for 6 h, followed by exposure to 100 μM H2O2 for 24 h to induce oxidative stress. Pre-treatment with NAC significantly increased intracellular GSH levels by up to two fold and prevented H2O2-induced intracellular redox imbalance, apoptosis and senescence. When critical-sized rat femur defects were filled with a collagen sponge containing fluorescent-labeled autologous BMSCs with or without NAC treatment, the number of apoptotic and surviving cells in the transplanted site after 3 days was significantly lower and higher in the NAC pre-treated group, respectively. By the 5th week, significantly enhanced new bone formation was observed in the NAC pre-treated group. These data suggest that pre-treatment of BMSCs with NAC before local transplantation enhances...Continue Reading

References

Jun 1, 1989·Acta Orthopaedica Scandinavica·H OhgushiA I Caplan
Mar 5, 2003·Cellular and Molecular Life Sciences : CMLS·M ZafarullahM Ahmad
May 22, 2004·Stem Cells·Joachim OswaldCarsten Werner
May 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ken SuzukiMagdi H Yacoub
Sep 18, 2004·The Journal of Biological Chemistry·Suparna QanungoAnna-Liisa Nieminen
Feb 24, 2005·Neuromuscular Disorders : NMD·Nicolas HolzerJacques Menetrey
Sep 28, 2005·The Journal of Investigative Dermatology·Rachael Clark, Thomas Kupper
Jun 5, 2007·The International Journal of Biochemistry & Cell Biology·Julia E RaftosPhilip W Kuchel
Mar 26, 2008·Journal of Dental Research·M YamadaT Ogawa
Apr 25, 2008·Biomaterials·Gert J MeijerClemens A van Blitterswijk
May 27, 2008·Journal of Dental Research·A ParanjpeA Jewett
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Jan 24, 2009·Redox Report : Communications in Free Radical Research·Masahiro YamadaTakahiro Ogawa
Mar 25, 2009·Journal of Translational Medicine·Jose-Ignacio AriasJaime Arias
May 16, 2009·Acta Biomaterialia·Masahiro Yamada, Takahiro Ogawa
Jul 15, 2009·Cell Death and Differentiation·M Maccarrone, B Brüne
Aug 12, 2009·Cell Research·Francesco P FiorentinoAntonio Giordano
Dec 15, 2010·Tissue Engineering. Part a·Corinna E ZimmermannHendrik Terheyden
Jan 7, 2011·Journal of Leukocyte Biology·Okan BastianTaco Blokhuis
May 5, 2011·Tissue Engineering. Part a·Florian BoukhechbaNathalie Rochet
Apr 26, 2012·Nature Reviews. Immunology·Katarina Le Blanc, Dimitrios Mougiakakos
Jul 11, 2012·Cell Transplantation·Darnell KaiglerWilliam V Giannobile
Jul 12, 2012·Journal of Tissue Engineering and Regenerative Medicine·Gregory TourIon Tcacencu
Nov 10, 2012·Journal of Prosthodontic Research·Hiroshi EgusaKentaro Akiyama
May 29, 2013·Biomaterials·Masahiro YamadaTakahiro Ogawa
Jun 5, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Meir LiebergallShaul Beyth
Sep 3, 2013·Antioxidants & Redox Signaling·Manish MittalAsrar B Malik
Jan 31, 2014·Journal of Tissue Engineering and Regenerative Medicine·Rainer Detsch, Aldo R Boccaccini
Apr 1, 2014·Brain and Behavior·Reza Bavarsad ShahripourAndrei V Alexandrov
Jul 23, 2014·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Soulafa A AlmazrooaSook-Bin Woo
Aug 30, 2014·Medicinal Research Reviews·Yunlong LeiCanhua Huang
Feb 6, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Darnell KaiglerWilliam V Giannobile
May 23, 2015·Journal of Immunology Research·Tracy K KovachQuanjun Cui

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Citations

Apr 3, 2020·Journal of Cellular Physiology·Mohammad PanahiEffat Alizadeh
May 8, 2020·International Journal of Molecular Medicine·Yang ZhouZhang Sun
Oct 23, 2019·International Journal of Molecular Sciences·Masahiro YamadaHiroshi Egusa
Oct 16, 2019·Biointerphases·Sudipto DattaPallab Datta
Jan 7, 2021·Stem Cell Reviews and Reports·Hesam Saghaei BagheriReza Rahbarghazi
Mar 9, 2021·Drug Discovery Today·Xin LiLing Ye
Jul 1, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Soo-Kyung JunHae-Hyoung Lee
Jun 3, 2021·International Journal of Molecular Sciences·Agnese GugliandoloFrancesca Diomede
Aug 9, 2021·Free Radical Biology & Medicine·Ziling WangYaping Wang

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