Preconditioning of bovine endothelial cells. The protective effect is mediated by an adenosine A2 receptor through a protein kinase C signaling pathway

Circulation Research
X ZhouM Ashraf

Abstract

We tested the hypothesis that anoxic preconditioning could protect coronary endothelial cells against anoxic and reoxygenation injury and that this preconditioning effect could be mediated by an adenosine A2 receptor via the protein kinase C (PKC) pathway. Cells were preconditioned with 10-minute anoxia and 10-minute reoxygenation and were then subjected to anoxia for 60 minutes, followed by 120 minutes of reoxygenation. In some groups, the preconditioning effect was prevented by 8-sulfophenyltheophylline (SPT [50 mumol/L], a nonselective adenosine receptor antagonist), or calphostin C (100 nmol/L, a PKC inhibitor). In other groups, 2-p-(2-carboxyethyl)phenethylamino-5'N-ethylcarboxyamido- adenosine (CGS-21680 [20 nmol/L], an adenosine A2 receptor agonist, R-(--)-N6-(2-phenylisopropyl)-adenosine (R-PIA [50 nmol/L], an adenosine A1 receptor agonist), or 4 beta-phorbol 12-myristate 13-acetate (PMA [100 nmol/L], a PKC activator) was given as a pretreatment to mimic the preconditioning effect. Endothelial cells were also pretreated with 100 nmol/L calphostin C to confirm whether inhibition of PKC can block the effects of adenosine A2 receptor activation by CGS-21680 on anoxia and reoxygenation injury. Preconditioning reduced LDH re...Continue Reading

References

Jul 1, 1990·Physiological Reviews·R A Olsson, J D Pearson
Jan 1, 1990·Journal of Molecular and Cellular Cardiology·R D LasleyR M Mentzer
Jul 1, 1989·Progress in Cardiovascular Diseases·L BelardinelliR M Berne
Jul 1, 1987·Naunyn-Schmiedeberg's Archives of Pharmacology·C Des Rosiers, S Nees
Jan 1, 1986·Annual Review of Physiology·P M VanhoutteD S Houston
Mar 1, 1993·Journal of Molecular and Cellular Cardiology·J D ThorntonJ M Downey

❮ Previous
Next ❯

Citations

Nov 1, 1996·Basic Research in Cardiology·Y LiuE Marban
Jun 19, 1997·The American Journal of Cardiology·J C Shryock, L Belardinelli
Mar 18, 2000·Pharmacology & Therapeutics·M E Olah, G L Stiles
Jun 6, 2000·Trends in Pharmacological Sciences·A Rubino, D M Yellon
Apr 17, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M AraiJ J Lemasters
Dec 6, 1997·Annals of Surgery·D R MeldrumA H Harken
Nov 6, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anuska V AndjelkovicRichard F Keep
Dec 7, 2013·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Nikhil KapoorMassoud A Leesar
Nov 1, 1996·Journal of Cardiovascular Pharmacology·A TosakiD K Das
Mar 17, 2005·Experimental Physiology·Carolina Carrasco-MartínAntonio J López-Farré
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D MerkusW M Chilian
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Thorsten Reffelmann, Robert A Kloner
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Taiji YamaguchiRonald J Korthuis
Nov 15, 1997·The American Journal of Physiology·A TosakiD K Das
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M SchäferT Noll

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.