PMID: 4889363Jan 1, 1968Paper

Precursors of the pyrimidine moiety of thiamine

The Biochemical Journal
P C Newell, R G Tucker

Abstract

1. A method was devised for obtaining the pyrimidine moiety of thiamine in a pure form after its excretion into the medium by de-repressed washed-cell suspensions of mutants of Salmonella typhimurium LT2. 2. By using amino acid-requiring mutants, this excretion of pyrimidine moiety was shown to be dependent on the presence of both methionine and glycine. 3. In the presence of either [Me-(14)C]methionine or [G-(14)C]methionine, methionine-requiring mutants did not incorporate radioactivity into the pyrimidine moiety. 4. In contrast, both [1-(14)C]glycine and [2-(14)C]glycine were incorporated into the pyrimidine moiety excreted by glycine-requiring mutants, and this occurred with little or no dilution of specific radioactivity. 5. The possible requirement for methionine as a cofactor and the significance of the incorporation of both carbon atoms of glycine are discussed.

Citations

Oct 26, 1971·European Journal of Biochemistry·J H Wittorf, C J Gubler
Jun 15, 2006·Annual Review of Microbiology·Diana M Downs
Sep 25, 1999·Canadian Journal of Microbiology·T Christian, D M Downs
Jan 21, 2003·Journal of Bacteriology·Glenn R Björk, Kristina Nilsson
Sep 8, 2010·The Journal of Biological Chemistry·Jennifer A LambrechtDiana M Downs
Nov 23, 1983·Biochimica Et Biophysica Acta·E BellionB R McKellar
Jun 1, 1976·Applied and Environmental Microbiology·H E Rubin, S H Love
Aug 1, 1994·Journal of Bacteriology·D M Downs, L Petersen
Oct 1, 1991·Journal of Bacteriology·D M Downs, J R Roth
Aug 1, 1975·Journal of Bacteriology·R D Mosteller, R V Goldstein
Sep 9, 2021·Chembiochem : a European Journal of Chemical Biology·Ying-Jie Sun, Liang Cheng

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