Predicted multiple selected reaction monitoring to screen activated drug-mediated modifications on human serum albumin

Analytical Biochemistry
Fumio OsakiTomoyuki Oe

Abstract

Metabolic activation of drugs frequently generates electrophilic products that may undergo covalent binding to biological macromolecules, such as proteins and DNA. The resulting covalent adducts are of considerable concern in drug discovery and development. Several strategies for assessing the potential risks of candidate drugs have been reported. Of these, glutathione trapping is the most commonly used method together with mass spectrometry. Furthermore, drug-mediated protein modifications have been studied using serum albumin and CYP enzymes to clarify target amino acids and mechanism-based inhibition, respectively. In this article, we introduce a practical way to screen drug-mediated protein modifications. The method, referred to as "predicted multiple selected reaction monitoring," is based on the selected reaction monitoring (SRM) strategy, but targets all possible chemically modified tryptic peptides. The creation of SRM lists may require patience; however, this strategy could facilitate more sensitive screening compared with the common strategy of data-dependent product ion scanning. Ketoprofen-N-hydroxysuccinimidyl ester (equivalent to glucuronide) and N-acetyl-p-benzoquinone imine (NAPQI) were allowed to react with hum...Continue Reading

References

May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A DingL Z Benet
Mar 20, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sebastien BolzeThierry Hulot
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Mar 8, 2005·Drug Metabolism Reviews·Shufeng ZhouYu-Zong Chen
Mar 15, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Zhengyin YanHua Zhong
Aug 30, 2005·Journal of Pharmacological and Toxicological Methods·S H DayM G Beconi
Oct 6, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hai-Zhi BuWilliam F Pool
Jun 7, 2006·Rapid Communications in Mass Spectrometry : RCM·Robert S PlumbJeremy K Nicholson
Mar 9, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Amit S KalgutkarKlaas Schildknegt
May 19, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Micaela C DamstenNico P E Vermeulen
Oct 27, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sandra OsbildEric Battaglia
Apr 20, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jianyao WangRasmy Talaat
Sep 3, 2010·Chemical Research in Toxicology·Alexandra M M AntunesM Matilde Marques
Nov 16, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jaana E LaineRisto O Juvonen
Jun 1, 2009·Proteomics. Clinical Applications·Rosalind E JenkinsB Kevin Park
Apr 2, 2011·Nature Reviews. Drug Discovery·B Kevin ParkThomas A Baillie
Jun 18, 2011·The Journal of Pharmacology and Experimental Therapeutics·Xiaoli MengB Kevin Park
Jul 26, 2011·Journal of Allergy·Itai ChipindaPaul D Siegel
Mar 1, 2012·The Journal of Pharmacology and Experimental Therapeutics·Sabah El-GhaieshDean J Naisbitt
Jul 10, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Sarim Dastgir, Gino G Lavoie
Sep 1, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Chitra SridarPaul F Hollenberg

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Citations

Mar 31, 2015·Advanced Drug Delivery Reviews·George LambrinidisAnna Tsantili-Kakoulidou
Oct 15, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Zari HooshyarNastaran Dianatnejad
Jun 17, 2020·Archives of Toxicology·Andrea HartwigGerhard Eisenbrand
Jun 10, 2017·Archives of Toxicology·Benjamin SachseBernhard H Monien

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