Predicted secreted protein analysis reveals synaptogenic function of Clstn3 during WAT browning and BAT activation in mice

Acta Pharmacologica Sinica
Shu-Qin ChenWei-Ping Jia

Abstract

Promoting white adipose tissue (WAT) browning and enhancing brown adipose tissue (BAT) activity are attractive therapeutic strategies for obesity and its metabolic complications. Targeting sympathetic innervation in WAT and BAT represents a promising therapeutic concept. However, there are few reports on extracellular microenvironment remodeling, especially changes in nerve terminal connections. Identifying the key molecules mediating the neuro-adipose synaptic junctions is a key point. In this study, we used bioinformatics methods to identify the differentially expressed predicted secreted genes (DEPSGs) during WAT browning and BAT activation. These DEPSGs largely reflect changes of cytokines, extracellular matrix remodeling, vascularization, and adipocyte-neuronal cross-talk. We then performed functional enrichment and cellular distribution specificity analyses. The upregulated and downregulated DEPDGs during WAT browning displayed a distinctive biological pattern and cellular distribution. We listed a cluster of adipocyte-enriched DEPSGs, which might participate in the cross-talk between mature adipocytes and other cells; then identified a synaptogenic adhesion molecule, Clstn3, as the top gene expressed enriched in both mat...Continue Reading

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Datasets Mentioned

BETA
GSE86338
GSE44059
GEO863338
GSE9954

Methods Mentioned

BETA
fluorescence-imaging
RNA-Seq

Software Mentioned

Phobius
SignalP4
SPOCTOPUS
GEO
EBSeq

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