Predicting adhesion and biofilm formation boundaries on stainless steel surfaces by five Salmonella enterica strains belonging to different serovars as a function of pH, temperature and NaCl concentration

International Journal of Food Microbiology
Juliana O MoraesMarciane Magnani

Abstract

This study aimed to assess the capability of 97 epidemic S. enterica strains belonging to 18 serovars to form biofilm. Five strains characterized as strong biofilm-producers, belonging to distinct serovars (S. Enteritidis 132, S. Infantis 176, S. Typhimurium 177, S. Heidelberg 281 and S. Corvallis 297) were assayed for adhesion/biofilm formation on stainless steel surfaces. The experiments were conducted in different combinations of NaCl (0, 2, 4, 5, 6, 8 and 10% w/v), pH (4, 5, 6 and 7) and temperatures (8 °C, 12 °C, 20 °C and 35 °C). Only adhesion was assumed to occur when S. enterica counts were ≥3 and <5 log CFU/cm2, whereas biofilm formation was defined as when the counts were ≥5 log CFU/cm2. The binary responses were used to develop models to predict the probability of adhesion/biofilm formation on stainless steel surfaces by five strains belonging to different S. enterica serovars. A total of 99% (96/97) of the tested S. enterica strains were characterized as biofilm-producers in the microtiter plate assays. The ability to form biofilm varied (P < 0.05) within and among the different serovars. Among the biofilm-producers, 21% (20/96), 45% (43/96), and 35% (34/96) were weak, moderate and strong biofilm-producers, respecti...Continue Reading

Citations

Nov 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriella SpenglerEnrique Domínguez-Álvarez
Feb 6, 2020·Canadian Journal of Microbiology·Emmanuel BumunangYan D Niu
Jan 21, 2020·Food Research International·Christina SakarikouEfstathios Giaouris
Nov 26, 2021·Journal of Applied Microbiology·Tomi ObeNikki W Shariat
Nov 26, 2020·Food Research International·Alexandra LianouKonstantinos P Koutsoumanis

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