Predicting chemotherapeutic drug combinations through gene network profiling

Scientific Reports
Thi Thuy Trang NguyenEe Sin Chen

Abstract

Contemporary chemotherapeutic treatments incorporate the use of several agents in combination. However, selecting the most appropriate drugs for such therapy is not necessarily an easy or straightforward task. Here, we describe a targeted approach that can facilitate the reliable selection of chemotherapeutic drug combinations through the interrogation of drug-resistance gene networks. Our method employed single-cell eukaryote fission yeast (Schizosaccharomyces pombe) as a model of proliferating cells to delineate a drug resistance gene network using a synthetic lethality workflow. Using the results of a previous unbiased screen, we assessed the genetic overlap of doxorubicin with six other drugs harboring varied mechanisms of action. Using this fission yeast model, drug-specific ontological sub-classifications were identified through the computation of relative hypersensitivities. We found that human gastric adenocarcinoma cells can be sensitized to doxorubicin by concomitant treatment with cisplatin, an intra-DNA strand crosslinking agent, and suberoylanilide hydroxamic acid, a histone deacetylase inhibitor. Our findings point to the utility of fission yeast as a model and the differential targeting of a conserved gene intera...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jan 8, 2000·Journal of Inorganic Biochemistry·J M MalingeM Leng
Jan 19, 2002·Genes & Development·Yumei LiStephen J Elledge
Oct 31, 2003·Molecular and Cellular Biology·Hui ZhaoPaul Russell
Sep 7, 2004·The Journal of Biological Chemistry·Izumi SugimotoMakoto Nakanishi
Feb 17, 2005·Bioinformatics·C Thach NguyenLouxin Zhang
Mar 7, 2006·Nature Reviews. Drug Discovery·Gergely SzakácsMichael M Gottesman
Apr 20, 2006·Microbiology·Tomoko IwakiKaoru Takegawa
Dec 19, 2006·Chemical Research in Toxicology·Michael D Wyatt, Douglas L Pittman
Apr 24, 2007·Nature Structural & Molecular Biology·Estelle NicolasShiv I S Grewal
Aug 19, 2007·Nature Reviews. Genetics·Andreas BeyerTrey Ideker
Jul 16, 2008·Nature Methods·David K BreslowJonathan S Weissman
Dec 17, 2008·Nature Methods·Ariel S SchwartzTrey Ideker
Apr 8, 2009·Cancer Chemotherapy and Pharmacology·Nina Saban, Maro Bujak
Jun 18, 2009·Biotechnology and Applied Biochemistry·Makoto Kawamukai
Jul 17, 2009·Molecular Biology of the Cell·Akhmed AslamG Sebastiaan Winkler
Oct 20, 2009·European Journal of Pharmacology·Emilia Wiechec, Lise Lotte Hansen
Feb 2, 2010·International Journal of Radiation Oncology, Biology, Physics·Sheetal SinghAndrew T Vaughan
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·J-H LeePaul A Marks
Nov 16, 2010·Nature Methods·Anastasia BaryshnikovaChad L Myers
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Apr 28, 2011·Anti-cancer Agents in Medicinal Chemistry·William Greenhalf, Amy Thomas
Jan 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Or ZukEric S Lander
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Jan 1, 2013·Molecular Cell·Aude GuénoléHaico van Attikum
Jan 18, 2013·BMC Systems Biology·Dario Ghersi, Mona Singh
Feb 26, 2013·Current Medicinal Chemistry·G L BerettaN Zaffaroni
May 30, 2013·American Society of Clinical Oncology Educational Book·Javier MunozRazelle Kurzrock

❮ Previous
Next ❯

Citations

Jan 26, 2016·Pharmacology & Therapeutics·Rebecca A Jackson, Ee Sin Chen
Mar 4, 2017·Microbial Cell Factories·Sean Qiu En LeeEe Sin Chen
Apr 21, 2018·Future Medicinal Chemistry·Zdeněk KejíkVladimír Král
Sep 12, 2017·Advanced Healthcare Materials·Cecilia SahlgrenNiklas Sandler
Aug 11, 2020·Oncotarget·Brandon Yi Da HoongEe Sin Chen
Oct 4, 2018·PeerJ·Siti Nurulnabila A RahamanChyan Leong Ng
Aug 17, 2016·BMC Systems Biology·Núria Ballber Torres, Claudio Altafini
Jul 14, 2018·Cellular and Molecular Life Sciences : CMLS·Ee Sin Chen
Jan 10, 2021·Diagnostics·Megan L HutchcraftJill M Kolesar
Jan 15, 2021·International Journal of Molecular Sciences·Kim Kiat LimEe Sin Chen
May 30, 2021·Nature Communications·Yuma IshigamiTsutomu Suzuki

❮ Previous
Next ❯

Methods Mentioned

BETA
chromosomal aberrations
xenografts

Software Mentioned

Excel
String

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

© 2022 Meta ULC. All rights reserved