Predicting Confined 1D Cell Migration from Parameters Calibrated to a 2D Motor-Clutch Model

Biophysical Journal
Louis S PrahlDavid J Odde

Abstract

Biological tissues contain micrometer-scale gaps and pores, including those found within extracellular matrix fiber networks, between tightly packed cells, and between blood vessels or nerve bundles and their associated basement membranes. These spaces restrict cell motion to a single-spatial dimension (1D), a feature that is not captured in traditional in vitro cell migration assays performed on flat, unconfined two-dimensional (2D) substrates. Mechanical confinement can variably influence cell migration behaviors, and it is presently unclear whether the mechanisms used for migration in 2D unconfined environments are relevant in 1D confined environments. Here, we assessed whether a cell migration simulator and associated parameters previously measured for cells on 2D unconfined compliant hydrogels could predict 1D confined cell migration in microfluidic channels. We manufactured microfluidic devices with narrow channels (60-μm2 rectangular cross-sectional area) and tracked human glioma cells that spontaneously migrated within channels. Cell velocities (vexp = 0.51 ± 0.02 μm min-1) were comparable to brain tumor expansion rates measured in the clinic. Using motor-clutch model parameters estimated from cells on unconfined 2D pla...Continue Reading

References

Jul 1, 1991·Biophysical Journal·P A DiMillaD A Lauffenburger
Mar 23, 1987·FEBS Letters·M CouéE D Korn
Nov 9, 1995·Nature·J E MolloyD C White
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Aug 12, 2000·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·U Novak, A H Kaye
Jan 12, 2005·Biochemistry·Takeshi SakamotoJames R Sellers
Apr 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Florence LefrancRobert Kiss
Jan 6, 2007·Journal of Neuropathology and Experimental Neurology·Hana L P HarpoldKristin R Swanson
Mar 12, 2008·Biochemical and Biophysical Research Communications·Tanmay P LeleDonald E Ingber
May 23, 2008·Molecular Biology of the Cell·Christopher BeadlePeter Canoll
Sep 26, 2008·Journal of Mathematical Biology·R RockneK R Swanson
Dec 17, 2008·Science·Clarence E Chan, David J Odde
Dec 17, 2008·The Journal of Cell Biology·Margaret L GardelClare M Waterman
Nov 17, 2009·Journal of Biomechanics·Jonathan StrickerMargaret L Gardel
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Daniel Irimia, Mehmet Toner
Aug 23, 2011·Cell·Melissa K GardnerDavid J Odde
Oct 19, 2011·The Journal of Pathology·Pavlo G GritsenkoPeter Friedl
Jan 6, 2012·Molecular Biology of the Cell·Sanja IvkovicSteven S Rosenfeld
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amit Pathak, Sanjay Kumar
Jun 19, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eric M BalzerKonstantinos Konstantopoulos
Aug 21, 2012·Current Biology : CB·Dominique SeetapunDavid J Odde
Jul 24, 2013·Annual Review of Cell and Developmental Biology·Sandrine Etienne-Manneville
Aug 6, 2013·Annual Review of Cell and Developmental Biology·Gaudenz DanuserAlex Mogilner
Oct 9, 2013·Lab on a Chip·Phrabha S RamanKonstantinos Konstantopoulos
Dec 7, 2013·Nature Communications·Kerry WilsonGuillaume Charras
Jan 28, 2014·Cellular and Molecular Bioengineering·Benjamin L Bangasser, David J Odde
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pei-Hsun WuDenis Wirtz
Mar 19, 2014·Methods in Enzymology·Louis S PrahlDavid J Odde
Apr 15, 2014·Cell·Kimberly M StrokaKonstantinos Konstantopoulos
May 6, 2014·Nature Materials·Alberto Elosegui-ArtolaPere Roca-Cusachs
Jun 21, 2014·Nature Reviews. Neuroscience·Vishnu Anand CuddapahHarald Sontheimer
Dec 2, 2014·Cellular and Molecular Bioengineering·Patricia M DavidsonJan Lammerding
Feb 14, 2015·Cell·Verena RuprechtCarl-Philipp Heisenberg
Feb 20, 2015·Nature Communications·Ovijit ChaudhuriDavid J Mooney
Mar 17, 2015·Nature Cell Biology·Martin BergertEwa K Paluch
Aug 19, 2015·Journal of Biomechanics·Kerianne E SteuckePatrick W Alford
Dec 8, 2015·Nature Cell Biology·Pablo VargasAna-Maria Lennon-Duménil
Jan 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Harrison V Prentice-MottJagesh V Shah
Feb 26, 2016·Molecular Biology of the Cell·Pascale MonzoMichael P Sheetz

❮ Previous
Next ❯

Citations

Sep 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Pierre Sens
Aug 28, 2020·Current Opinion in Oncology·Tiffanie ChouleurAndreas Bikfalvi
Mar 11, 2021·Cell Reports·Benjamin YeomanAdam J Engler
Nov 1, 2021·International Journal for Numerical Methods in Biomedical Engineering·Chia-Yu ChangPo-Jen Shih

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
nucleotide exchange
GTPase

Software Mentioned

MATLAB
NIS
Elements Advanced Research
AutoCAD

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

BioRxiv : the Preprint Server for Biology
Louis S PrahlDavid J Odde
The Journal of Cell Biology
Wei-Chien HungKonstantinos Konstantopoulos
Annals of Biomedical Engineering
Horacio M EstabridisDavid J Odde
Cellular and Molecular Bioengineering
Benjamin L Bangasser, David J Odde
© 2021 Meta ULC. All rights reserved