Predicting evolutionary potential: in vitro evolution accurately reproduces natural evolution of the tem beta-lactamase.

Genetics
Miriam Barlow, Barry G Hall

Abstract

To evaluate the validity of our in vitro evolution method as a model for natural evolutionary processes, the TEM-1 beta-lactamase gene was evolved in vitro and was selected for increased resistance to cefotaxime, cefuroxime, ceftazadime, and aztreonam, i.e., the "extended-spectrum" phenotype. The amino acid substitutions recovered in 10 independent in vitro evolvants were compared with the amino acid substitutions in the naturally occurring extended-spectrum TEM alleles. Of the nine substitutions that have arisen multiple times in naturally occurring extended-spectrum TEM alleles, seven were recovered multiple times in vitro. We take this result as evidence that our in vitro evolution technique accurately mimics natural evolution and can therefore be used to predict the results of natural evolutionary processes. Additionally, our results predict that a phenotype not yet observed among TEM beta-lactamases in nature-resistance to cefepime-is likely to arise in nature.

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Citations

Sep 30, 2008·Journal of Molecular Biology·David C MarcianoTimothy Palzkill
Jul 2, 2008·Journal of Molecular Evolution·Mia H C Baquirin, Miriam Barlow
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·S HaeggmanS Brisse
Jul 8, 2008·Journal of Inorganic Biochemistry·Peter Oelschlaeger
Apr 19, 2003·Genetics·Miriam Barlow, Barry G Hall
Jul 13, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Weiqun PengDavid A Kessler
Jul 16, 2008·Antimicrobial Agents and Chemotherapy·Joanna E Mroczkowska, Miriam Barlow
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Mar 8, 2008·Emerging Infectious Diseases·Miriam BarlowFred C Tenover
Mar 21, 2008·Nature·Daniela RöthlisbergerDavid Baker
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Kerry J WelshJohn E McGowan
Apr 30, 2008·Antimicrobial Agents and Chemotherapy·Angela NovaisJuan Carlos Galán
Nov 26, 2015·Antimicrobial Agents and Chemotherapy·Marius LinkeviciusDan I Andersson
Apr 22, 2004·Nature Reviews. Microbiology·Barry G Hall
May 26, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Barry G Hall, Miriam Barlow
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Mar 17, 2011·PLoS Genetics·Merijn L M SalverdaJ Arjan G M de Visser

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