Predicting Ligand-Free Cell Attachment on Next-Generation Cellulose-Chitosan Hydrogels

ACS Omega
Marcus A JohnsRam I Sharma

Abstract

There is a growing appreciation that engineered biointerfaces can regulate cell behaviors, or functions. Most systems aim to mimic the cell-friendly extracellular matrix environment and incorporate protein ligands; however, the understanding of how a ligand-free system can achieve this is limited. Cell scaffold materials comprised of interfused chitosan-cellulose hydrogels promote cell attachment in ligand-free systems, and we demonstrate the role of cellulose molecular weight, MW, and chitosan content and MW in controlling material properties and thus regulating cell attachment. Semi-interpenetrating network (SIPN) gels, generated from cellulose/ionic liquid/cosolvent solutions, using chitosan solutions as phase inversion solvents, were stable and obviated the need for chemical coupling. Interface properties, including surface zeta-potential, dielectric constant, surface roughness, and shear modulus, were modified by varying the chitosan degree of polymerization and solution concentration, as well as the source of cellulose, creating a family of cellulose-chitosan SIPN materials. These features, in turn, affect cell attachment onto the hydrogels and the utility of this ligand-free approach is extended by forecasting cell attac...Continue Reading

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Citations

Oct 24, 2019·Anais Da Academia Brasileira De Ciências·Fernando GalembeckLeandra P Santos
Jan 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·James C CourtenayJanet L Scott
May 7, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Marcus A JohnsStephen J Eichhorn
Mar 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·James C CourtenayJanet L Scott
Oct 6, 2021·Chemical Society Reviews·Saixing TangWang Zhang Yuan

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atomic force microscopy
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