Predicting myofiber size with electrical impedance myography: A study in immature mice

Muscle & Nerve
Kush KapurSeward B Rutkove

Abstract

Electrical impedance can be used to estimate cellular characteristics. We sought to determine whether it could be used to approximate myofiber size using standard prediction modeling approaches. Forty-four C57BL/6J wild-type immature mice of varying ages underwent electrical impedance myography (EIM) with a needle electrode array placed in the gastrocnemius. Animals were then humanely killed and muscle fixed, stained, and myofiber size quantified. Two different statistical prediction models were then applied. Impedance parameters showed major variation with increasing myofiber size. The prediction models based on EIM data alone were able to predict fiber size, with errors in the range of ±69.05-78.44 µm2 (16.19%-18.40% with respect to the average myofiber size). By using well-established statistical models, EIM data alone can provide a satisfactory estimate of myofiber size. Additional study of this approach for approximating myofiber size without the requirement of removing tissue for histological analysis is warranted. Muscle Nerve, 2018.

References

Jul 3, 2009·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Mohammad A Ahad, Seward B Rutkove
Jun 8, 2012·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Seward B RutkoveJeremy M Shefner
Mar 1, 2012·Journal of the Royal Statistical Society. Series B, Statistical Methodology·Robert TibshiraniRyan J Tibshirani
Nov 5, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Benjamin Sanchez, Seward B Rutkove
Jan 12, 2017·Annals of Neurology·Seward B RutkoveBasil T Darras

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Citations

Oct 7, 2018·Cold Spring Harbor Perspectives in Medicine·Seward B Rutkove, Benjamin Sanchez
Sep 19, 2019·Muscle & Nerve·Joanne Clark-MatottSeward B Rutkove
Apr 21, 2020·Frontiers in Physiology·Carson SempleMarie Mortreux
Nov 2, 2020·The Journal of Physiological Sciences : JPS·Hiroki SatoKozo Hanayama
Jan 16, 2021·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Benjamin SanchezCynthia M Furse

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