Apr 30, 2020

Antibody repertoire and gene expression dynamics of diverse human B cell states during affinity maturation.

BioRxiv : the Preprint Server for Biology
H. W. KingLouisa K James

Abstract

In response to antigen challenge, B cells clonally expand, undergo selection and differentiate to produce mature B cell subsets and high affinity antibodies. However, the interplay between dynamic B cell states and their antibody-based selection is challenging to decipher in primary human tissue. We have applied an integrated analysis of bulk and single-cell antibody repertoires paired with single-cell transcriptomics of human B cells undergoing affinity maturation. We define unique gene expression and antibody repertoires of known and novel B cell states, including a pre-germinal centre state primed to undergo class switch recombination. We dissect antibody class-dependent gene expression of germinal centre and memory B cells to find that class switching prior to germinal centre entry can have significant implications for the capacity of B cells to undergo antibody-based selection and differentiate. Together, our analyses provide unprecedented resolution into the gene expression and selection dynamics that shape B cell-mediated immunity.

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Mentioned in this Paper

Extracellular Matrix
MHC binding peptide
Neural Network Simulation
Alleles
Ligands
Computed (Procedure)
Peptide/MHC Complex

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