Predicting Radiosensitivity with Gamma-H2AX Foci Assay after Single High-Dose-Rate and Pulsed Dose-Rate Ionizing Irradiation

Radiation Research
Bregje van OorschotNicolaas A P Franken

Abstract

Gamma-H2AX foci detection is the standard method to quantify DNA double-strand break (DSB) induction and repair. In this study, we investigated the induction and decay of γ-H2AX foci of different tumor cell lines and fibroblasts with known mutations in DNA damage repair genes, including ATM, LigIV, DNA-PKcs, Rad51 and Rad54. A radiation dose of 2.4 Gy was used for either an acute single high-dose-rate (sHDR) exposure or a pulsed dose-rate (pDR) exposure over 24 h. The number of γ-H2AX foci was determined at 30 min and 24 h after sHDR irradiation and directly after pDR irradiation. In a similar manner, γ-H2AX foci were also examined in lymphocytes of patients with differences in normal tissue toxicity after a total radiation dose of 1 Gy. In an initial count of the number of foci 30 min after sHDR irradiation, repair-proficient cell types could not be distinguished from repair-deficient cell types. However at 24 h postirradiation, while we observed a large decrease in foci numbers in NHEJ-proficient cells, the amount of γ-H2AX foci in cell types with mutated NHEJ repair remained at high levels. Except for IRS-1SF cells, HR-deficient cell types eventually did show a moderate decrease in foci number over time, albeit to a lesser e...Continue Reading

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Citations

Jun 6, 2016·Radiation and Environmental Biophysics·Daniel G AchelJohn M Akudugu
Apr 13, 2018·Annals of the ICRP·P RajaramanA Wojcik
Oct 21, 2017·Radiation Research·Sofia D'AbrantesStanley W Botchway
Jul 28, 2019·International Journal of Radiation Biology·Maria GomolkaSisko Salomaa
Mar 16, 2021·Dose-response : a Publication of International Hormesis Society·Ekaterina NikolovaVladimir Neychev
Nov 14, 2021·European Journal of Nuclear Medicine and Molecular Imaging·S SchumannU Eberlein

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