Predicting the accumulation of storage compounds by Rhodococcus jostii RHA1 in the feast-famine growth cycles using genome-scale flux balance analysis

PloS One
Mohammad Tajparast, Dominic Frigon

Abstract

Feast-famine cycles in biological wastewater resource recovery systems select for bacterial species that accumulate intracellular storage compounds such as poly-β-hydroxybutyrate (PHB), glycogen, and triacylglycerols (TAG). These species survive better the famine phase and resume rapid substrate uptake at the beginning of the feast phase faster than microorganisms unable to accumulate storage. However, ecophysiological conditions favouring the accumulation of either storage compounds remain to be clarified, and predictive capabilities need to be developed to eventually rationally design reactors producing these compounds. Using a genome-scale metabolic modelling approach, the storage metabolism of Rhodococcus jostii RHA1 was investigated for steady-state feast-famine cycles on glucose and acetate as the sole carbon sources. R. jostii RHA1 is capable of accumulating the three storage compounds (PHB, TAG, and glycogen) simultaneously. According to the experimental observations, when glucose was the substrate, feast phase chemical oxygen demand (COD) accumulation was similar for the three storage compounds; when acetate was the substrate, however, PHB accumulation was 3 times higher than TAG accumulation and essentially no glycoge...Continue Reading

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Citations

Sep 13, 2020·Applied Microbiology and Biotechnology·Martina CappellettiDavide Zannoni
May 20, 2020·Journal of Mathematical Biology·David S Tourigny
Apr 7, 2021·Biotechnology Advances·Youxiang Liang, Huimin Yu

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Methods Mentioned

BETA
GAM

Software Mentioned

openFLUX
Macrobal
FBA
iMS2Flux
MOMA
MATLAB

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