Prediction and characterization of protein-protein interaction network in Bacillus licheniformis WX-02

Scientific Reports
Yi-Chao HanLing-Ling Chen

Abstract

In this study, we constructed a protein-protein interaction (PPI) network of B. licheniformis strain WX-02 with interolog method and domain-based method, which contained 15,864 edges and 2,448 nodes. Although computationally predicted networks have relatively low coverage and high false-positive rate, our prediction was confirmed from three perspectives: local structural features, functional similarities and transcriptional correlations. Further analysis of the COG heat map showed that protein interactions in B. licheniformis WX-02 mainly occurred in the same functional categories. By incorporating the transcriptome data, we found that the topological properties of the PPI network were robust under normal and high salt conditions. In addition, 267 different protein complexes were identified and 117 poorly characterized proteins were annotated with certain functions based on the PPI network. Furthermore, the sub-network showed that a hub protein CcpA jointed directly or indirectly many proteins related to γ-PGA synthesis and regulation, such as PgsB, GltA, GltB, ProB, ProJ, YcgM and two signal transduction systems ComP-ComA and DegS-DegU. Thus, CcpA might play an important role in the regulation of γ-PGA synthesis. This study th...Continue Reading

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Methods Mentioned

BETA
two-hybrid
tandem affinity
MDS
RNA-seq
ssRNA-seq

Software Mentioned

TSN
HMMER
Cytoscape
PCD
BLASTP

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