Prediction and identification of B cell epitopes derived from EWS/FLI-l fusion protein of Ewing's sarcoma.

Medical Oncology
Huiwen LiuKai Cao

Abstract

To predict B cell epitope of Ewing's sarcoma EWS/FLI-l fusion protein and to analyze its antigenicity and immunogenicity. Comprehensive algorithms were applied to predict the possible B cell epitopes of EWS/FLI-l fusion protein. High-performance liquid chromatography (HPLC) and mass spectrometry (MS) analysis were performed to identify the synthesized epitope peptides, ELISA assays and Western blot to detect the antigenicity, and the immunogenicity of epitope peptides. Three B cell epitopes were screened out, and HPLC and MS analysis confirmed all three synthesized epitope peptides were demandable. ELISA assays verified all three epitope peptides could prime intense antigen-antibody reaction and induce ideal antibody titers after immunization to the New Zealand white rabbit. However, Western blot confirmed that antiserum of one of these epitope peptides could not recognize EWS/FLI-1 protein. Two B cell epitopes, PQDGNKPTETSQPQ and DPDEVARRWGQRKS, derived from EWS/FLI-l protein, are identified to have potential antigenicity and immunogenicity.

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Mar 1, 1988·Computer Applications in the Biosciences : CABIOS·B A Jameson, H Wolf
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 7, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·E de Alava, W L Gerald
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E H NardinK Rose
Oct 19, 2001·Oncogene·A Arvand, C T Denny
Apr 5, 2002·Journal of Virology·Anne-Kathrin ZaissDaniel A Muruve
Mar 26, 2003·Medical and Pediatric Oncology·Carlos Rodriguez-GalindoAlberto S Pappo
Apr 12, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naveen K DakappagariPravin T P Kaumaya
Sep 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·David Eisenberg
Jun 9, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Howard A ChanskyLiu Yang
Sep 2, 2004·Nature Medicine·Steven A RosenbergNicholas P Restifo
Mar 23, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lisa M BarkerDouglas S Hawkins
Apr 2, 2005·Nature Reviews. Immunology·Jacques Banchereau, A Karolina Palucka
Jul 5, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Danila ValmoriMaha Ayyoub
Jul 16, 2005·Pediatric Hematology and Oncology·Friederike Meyer-WentrupStefan Burdach
Jun 20, 2008·Clinical Orthopaedics and Related Research·Wei GuoHui Zhao
May 21, 2009·Journal of the American Chemical Society·Cheryl PaesBenjamin J Doranz
Mar 24, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marie-Cecile Le DeleyHeinrich Kovar
Sep 8, 2010·The New England Journal of Medicine·Philip W KantoffUNKNOWN IMPACT Study Investigators
Oct 28, 2010·Methods in Molecular Biology·Sinéad T Loughran, Dermot Walls
Feb 1, 2012·Cancer Immunology, Immunotherapy : CII·Chun-Jian QiKe-Qing Qian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.