PMID: 7517173Jul 1, 1994Paper

Prediction of accumulation of 131I-labelled meta-iodobenzylguanidine in neuroblastoma cell lines by means of reverse transcription and polymerase chain reaction

British Journal of Cancer
R J MairsA Barrett

Abstract

Radiolabelled meta-iodobenzylguanidine (mIBG) currently provides one of the most promising options for targeted radiotherapy of neuroblastoma. No means currently exists for prediction of mIBG uptake in tumour cells of individual patients other than semiquantitative inferences from diagnostic scanning which depend on the continued existence of a macroscopic tumour mass. A biological rapid assay which could be applied at initial biopsy would be invaluable in selecting patients for therapeutic strategies which incorporate radiolabelled mIBG. We have assessed the expression of the noradrenaline transporter gene in six human neuroblastoma cell lines and in three non-neural crest-derived cell lines using reverse transcription followed by the polymerase chain reaction. Transcription of this gene was observed in five out of six neuroblastoma cell lines but in none of the control cells. A highly significant correlation was established (P < 0.01) between gene expression and active cellular accumulation of mIBG. It is suggested that semiquantitative evaluation of noradrenaline transporter gene transcripts may be predictive of mIBG uptake by tumours in vivo.

Citations

Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Robert J MairsMarie Boyd
Jul 23, 2008·The Journal of Pharmacy and Pharmacology·Marie BoydRobert J Mairs
Dec 7, 2000·Medical and Pediatric Oncology·S CunninghamT E Wheldon
Oct 10, 2008·The British Journal of Radiology·A G McCluskeyR J Mairs
Sep 2, 2014·Pediatric Blood & Cancer·Keri A StrebyTimothy P Cripe
Oct 19, 2017·European Journal of Nuclear Medicine and Molecular Imaging·Jennifer E GainsMark N Gaze
Jul 17, 2013·European Journal of Nuclear Medicine and Molecular Imaging·Wolfgang Peter FendlerThomas Pfluger
Jun 16, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Katherine K MatthayJohn Babich
Sep 20, 2001·Bioconjugate Chemistry·G VaidyanathanM R Zalutsky
Jan 22, 2004·Seminars in Nuclear Medicine·Uwe HaberkornMichael Eisenhut

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