Prediction of chemical respiratory sensitizers using GARD, a novel in vitro assay based on a genomic biomarker signature

PloS One
Andy ForrerydMalin Lindstedt

Abstract

Repeated exposure to certain low molecular weight (LMW) chemical compounds may result in development of allergic reactions in the skin or in the respiratory tract. In most cases, a certain LMW compound selectively sensitize the skin, giving rise to allergic contact dermatitis (ACD), or the respiratory tract, giving rise to occupational asthma (OA). To limit occurrence of allergic diseases, efforts are currently being made to develop predictive assays that accurately identify chemicals capable of inducing such reactions. However, while a few promising methods for prediction of skin sensitization have been described, to date no validated method, in vitro or in vivo, exists that is able to accurately classify chemicals as respiratory sensitizers. Recently, we presented the in vitro based Genomic Allergen Rapid Detection (GARD) assay as a novel testing strategy for classification of skin sensitizing chemicals based on measurement of a genomic biomarker signature. We have expanded the applicability domain of the GARD assay to classify also respiratory sensitizers by identifying a separate biomarker signature containing 389 differentially regulated genes for respiratory sensitizers in comparison to non-respiratory sensitizers. By usi...Continue Reading

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Citations

Nov 1, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sander DikJanine Ezendam
Jan 27, 2017·International Journal of Molecular Sciences·Anki Malmborg, Carl A K Borrebaeck
Apr 16, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kazuto NaritaHiroshi Itagaki
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Jun 18, 2021·Regulatory Toxicology and Pharmacology : RTP·Emanoela Lundgren TháDaniela Morais Leme

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Datasets Mentioned

BETA
E-MEXP-3773

Methods Mentioned

BETA
Assay
flow cytometry
PCA
FCS

Software Mentioned

Epithelix
R
Bioconductor
FACS Diva
e1071
GARD
MetaCore
UPC
sva
ROCR

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