Prediction of delivery of organic aerosols onto air-liquid interface cells in vitro using an electrostatic precipitator

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Zechen YuHuanhuan Jiang

Abstract

To better characterize biological responses to atmospheric organic aerosols, the efficient delivery of aerosol to in vitro lung cells is necessary. In this study, chamber generated secondary organic aerosol (SOA) entered the commercialized exposure chamber (CULTEX® Radial Flow System Compact) where it interfaced with an electrostatic precipitator (ESP) (CULTEX® Electrical Deposition Device) and then deposited on a particle collection plate. This plate contained human lung cells (BEAS-2B) that were cultured on a membrane insert to produce an air-liquid interface (ALI). To augment in vitro assessment using the ESP exposure device, the particle dose was predicted for various sampling parameters such as particle size, ESP deposition voltage, and sampling flowrate. The dose model was evaluated against the experimental measured mass of collected airborne particles. The high flowrate used in this study increased aerosol dose but failed to achieve cell stability. For example, RNA in the ALI BEAS-2B cells in vitro was stable at 0.15L/minute but decayed at high flowrates. The ESP device and the resulting model were applied to in vitro studies (i.e., viability and IL-8 expression) of toluene SOA using ALI BEAS-2B cells with a flowrate of ...Continue Reading

Citations

Sep 19, 2018·Environmental Science and Pollution Research International·Jovile RaudoniuteRuta Aldonyte
Jul 14, 2021·Advanced Drug Delivery Reviews·Mohammed Ali SeloCarsten Ehrhardt
Nov 20, 2021·The Journal of Physical Chemistry. a·Zechen YuAzad Madhu

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