Prediction of HPLC retention times of tebipenem pivoxyl and its degradation products in solid state by applying adaptive artificial neural network with recursive features elimination

Talanta
Mikołaj MizeraJudyta Cielecka-Piontek

Abstract

A sensitive and fast HPLC method using ultraviolet diode-array detector (DAD)/electrospray ionization tandem mass spectrometry (Q-TOF-MS/MS) was developed for the determination of tebipenem pivoxyl and in the presence of degradation products formed during thermolysis. The chromatographic separations were performed on stationary phases produced in core-shell technology with particle diameter of 5.0 µm. The mobile phases consisted of formic acid (0.1%) and acetonitrile at different ratios. The flow rate was 0.8 mL/min while the wavelength was set at 331 nm. The stability characteristics of tebipenem pivoxyl were studied by performing stress tests in the solid state in dry air (RH=0%) and at an increased relative air humidity (RH=90%). The validation parameters such as selectivity, accuracy, precision and sensitivity were found to be satisfying. The satisfied selectivity and precision of determination were obtained for the separation of tebipenem pivoxyl from its degradation products using a stationary phase with 5.0 µm particles. The evaluation of the chemical structure of the 9 degradation products of tebipenem pivoxyl was conducted following separation based on the stationary phase with a 5.0 µm particle size by applying a Q-TO...Continue Reading

References

Jun 21, 2005·Chemical Research in Toxicology·H LiY Z Chen
May 10, 2007·Drugs·George G ZhanelJames A Karlowsky
Aug 24, 2011·Antimicrobial Agents and Chemotherapy·Krisztina M Papp-WallaceRobert A Bonomo
Sep 1, 2011·Analytical and Bioanalytical Chemistry·Bogusław Buszewski, Sylwia Noga
Jan 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Przemysław ZalewskiJudyta Cielecka-Piontek
Jun 6, 2014·ACS Medicinal Chemistry Letters·Sheo B SinghDeborah A Nicoll-Griffith
Nov 18, 2014·Chromatographia·Przemysław ZalewskiJudyta Cielecka-Piontek

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