Prediction of itching diagnostic marker through RNA sequencing of contact hypersensitivity and skin scratching stimulation mice models

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Young-Won KimJae-Hong Ko

Abstract

Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a , Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequenc...Continue Reading

References

Jan 2, 2003·QJM : Monthly Journal of the Association of Physicians·R TwycrossZ Zylicz
Dec 19, 2003·Nucleic Acids Research·Todd W HarrisLincoln D Stein
Jun 12, 2004·The Journal of Investigative Dermatology·Xu-Ping WangEhrhardt Proksch
Mar 3, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Won-Sik ShimUhtaek Oh
Jun 29, 2007·Acta Dermato-venereologica·Sonja StänderJeffrey D Bernhard
Jan 17, 2009·Neuroscience and Biobehavioral Reviews·Martin Schmelz
Feb 1, 2012·PLoS Computational Biology·Xinan YangYves A Lussier
Apr 28, 2012·Bioinformatics·David S DeLucaGad Getz
Aug 31, 2012·The Journal of Investigative Dermatology·Tetsuya HondaKenji Kabashima
Dec 12, 2012·The Journal of Investigative Dermatology·Heather M McGeeValerie Horsley
Oct 30, 2014·Nucleic Acids Research·Fiona CunninghamPaul Flicek
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Nov 25, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Xuming Zhang
Oct 4, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lu SunRosa Puertollano
May 14, 2016·Journal of Cell Communication and Signaling·Laure Rittié
May 21, 2016·Wiley Interdisciplinary Reviews. RNA·Radmila HrdlickovaBin Tian
Apr 13, 2017·Neuroscience Bulletin·Peng-Bo JingYong-Jing Gao
Dec 29, 2017·Neuroscience Bulletin·Carlene MooreWolfgang B Liedtke

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq

Software Mentioned

SPSS Statistics
Sailfish
Ensembl
ENVIGO
RNA
SeQC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.