Prediction of KIT mutation in gastrointestinal stromal tumors by the immunoprofile of the tumor cells

Journal of the Formosan Medical Association = Taiwan Yi Zhi
Chu-Chung ChouChin-Yuan Tzen

Abstract

Human KIT protooncogene is the cellular homolog of v-kit from the Hardy-Zuckerman 4 feline sarcoma virus, and encodes a 145-kDa type III tyrosine kinase growth factor receptor that is often mutated in gastrointestinal stromal tumors (GIST). Standardized mutation analysis is not available in many countries; therefore, we aimed to determine if the presence of KIT mutation in GIST can be predicted by the immunoprofile of the tumor cells. One hundred and forty-nine GIST were subjected to mutation analysis for KIT and immunohistochemical analysis for the expression of CD117, CD34, alpha-smooth muscle actin (SMA), and S100 protein. Mutation and immunohistochemistry data were correlated. KIT mutation rates were higher in certain immunoprofile subsets of GIST than in GIST in general. Compared with the overall mutation rate of KIT (70%), all GIST with CD117+ and S100+ had > 80% probability of harboring mutated KIT, and the subset with additional CD34+ and SMA- had a mutation rate of 88%. The overall KIT mutation rate in CD117- GIST was 31%. However, the probability of KIT mutation in CD117- GIST with CD34+SMA+S100-, CD34-SMA-S100+, and CD34+SMA-S100+ was 100%, 100%, and 67%, respectively. Compared with the overall mutation rate (8.7%) o...Continue Reading

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Citations

Mar 29, 2014·World Journal of Surgical Oncology·Liang Zong, Ping Chen
Jan 28, 2012·La Presse médicale·Sakina SekkateHassan Errihani
Jun 4, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Niraj KumariNarendra Krishnani
Feb 22, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Peter BirnerSebastian F Schoppmann

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