Prediction of naphthalene bioaccumulation using an adipocyte cell line model

Biotechnology Progress
Kwanchanok Viravaidya, Michael L Shuler

Abstract

A long-term goal of this research is to develop an in vitro model to study the metabolism, distribution, and fate of chemicals or pharmaceuticals in animals and humans. An important component of such a system is an in vitro model to study bioaccumulation of specific chemicals in adipose tissue. Due to the difficulties in maintaining primary adipocytes in culture and conducting reproducible experiments, transformed adipocyte cell lines have been used as an alternative. In this paper, several rodent preadipocyte cell lines (3T3-L1, 3T3-F442A, and TA1 cells) that differentiate into adipocytes when exposed to the appropriate stimuli are tested as an investigative tool to study naphthalene accumulation. The in vitro model is tested by comparison of its performance to that of primary adipocytes. All the experimental evidence supports the hypothesis that naphthalene accumulation is primarily dependent on the level of intracellular lipid. Furthermore, the level of naphthalene bioaccumulation is linearly correlated with the amount of triglyceride content with the slope of 37.7 +/- 0.5 microg of naphthalene/(mg of triglyceride). Indomethacin/dexamethasone/insulin are shown to be more effective in promoting preadipocyte differentiation th...Continue Reading

Citations

Dec 17, 2015·Environmental Technology·Hakan ÇelebiDelia Teresa Sponza
Apr 17, 2014·Journal of Biomedical Materials Research. Part a·George E AninweneThomas J Webster
Mar 7, 2017·Biotechnology Progress·Abdollah MoslehRobert Beitle
May 12, 2004·Biotechnology and Bioengineering·Kwanchanok ViravaidyaMichael L Shuler
Mar 3, 2020·Journal of Applied Microbiology·S A YounisH N Nassar
Oct 16, 2014·Environmental Engineering Science·Valdis Krumins, Donna E Fennell

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