Prediction of novel isoforms of the mouse Mpv17l protein

Genome Génome / Conseil National De Recherches Canada
Charalambos MagoulasLuis Lopez de Heredia

Abstract

The Mpv17l protein has two isoforms, M-LPL and M-LPS, which both regulate the production of reactive oxygen species (ROS) and protect against mitochondrial oxidative stress and apoptosis. M-LPL is ubiquitously expressed, while M-LPS is expressed mostly in the kidney of aged animals. We identified a variety of transcripts of the Mpv17l gene that could encode novel isoforms by mapping expressed sequence tags on the mouse M-LP genomic locus. We analysed the expression and evolutionary conservation of a novel Mpv17l transcript (mpv17l-002) that is predicted to encode a new protein, termed M-LP2. The isoform M-LP2 has the full length of M-LPS plus six amino acids at the end of its amino terminus, which could be encoded by an alternative 5'-flanking sequence. We show that the mRNA of M-LP2 has a different pattern of expression than the mRNA of M-LPS, and sequences of both transcripts are conserved in the rodent genome; however, neither of these isoforms is detected in the human genome. These observations suggest that the functions of certain M-LP isoforms are tissue- and species-specific, implying that their potential involvement in ROS metabolism may be redundant or may be complemented by other members of the Mpv17 family.

References

Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Daiya Takai, Peter A Jones
Feb 8, 2005·Journal of Molecular Evolution·Anton Nekrutenko
Apr 25, 2006·Biochemical and Biophysical Research Communications·Reiko IidaKoichiro Kishi
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Sep 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stefanie KrickErwin P Böttinger

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Citations

Sep 10, 2016·Mediators of Inflammation·Agnieszka LobodaJozef Dulak

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