Prediction of optimal contrast times post-imaging agent administration to inform personalized fluorescence-guided surgery.

Journal of Biomedical Optics
Negar SadeghipourKenneth M Tichauer

Abstract

Fluorescence guidance in cancer surgery (FGS) using molecular-targeted contrast agents is accelerating, yet the influence of individual patients' physiology on the optimal time to perform surgery post-agent-injection is not fully understood. Develop a mathematical framework and analytical expressions to estimate patient-specific time-to-maximum contrast after imaging agent administration for single- and paired-agent (coadministration of targeted and control agents) protocols. The framework was validated in mouse subcutaneous xenograft studies for three classes of imaging agents: peptide, antibody mimetic, and antibody. Analytical expressions estimating time-to-maximum-tumor-discrimination potential were evaluated over a range of parameters using the validated framework for human cancer parameters. Correlations were observed between simulations and matched experiments and metrics of tumor discrimination potential (p  <  0.05). Based on human cancer physiology, times-to-maximum contrast for peptide and antibody mimetic agents were <200  min, >15  h for antibodies, on average. The analytical estimates of time-to-maximum tumor discrimination performance exhibited errors of <10  %   on average, whereas patient-to-patient variance is...Continue Reading

Citations

Jul 20, 2021·Frontiers in Oncology·Servando Hernandez VargasAli Azhdarinia

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
fluorescence imaging
saturation binding
imaging techniques
biopsy

Software Mentioned

Graphpad Prism
MATLAB

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.