PMID: 6167991Jun 1, 1981Paper

Prediction of protein antigenic determinants from amino acid sequences

Proceedings of the National Academy of Sciences of the United States of America
T P Hopp, K R Woods

Abstract

A method is presented for locating protein antigenic determinants by analyzing amino acid sequences in order to find the point of greatest local hydrophilicity. This is accomplished by assigning each amino acid a numerical value (hydrophilicity value) and then repetitively averaging these values along the peptide chain. The point of highest local average hydrophilicity is invariably located in, or immediately adjacent to, an antigenic determinant. It was found that the prediction success rate depended on averaging group length, with hexapeptide averages yielding optimal results. The method was developed using 12 proteins for which extensive immunochemical analysis has been carried out and subsequently was used to predict antigenic determinants for the following proteins: hepatitis B surface antigen, influenza hemagglutinins, fowl plague virus hemagglutinin, human histocompatibility antigen HLA-B7, human interferons, Escherichia coli and cholera enterotoxins, ragweed allergens Ra3 and Ra5, and streptococcal M protein. The hepatitis B surface antigen sequence was synthesized by chemical means and was shown to have antigenic activity by radioimmunoassay.

References

Mar 1, 1978·Immunochemistry·F C Westall, M Thompson
Nov 16, 1976·Biochemistry·F R Maxfield, H A Scheraga
Mar 25, 1975·Biochemistry·L E MoleJ D Capra
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M So, B J McCarthy
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G D Rose, S Roy
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R ArnonL Chedid
Dec 9, 1980·Biochemistry·D G KlapperJ D Capra

❮ Previous
Next ❯

Citations

Dec 10, 2013·Journal of Proteome Research·Mohammad T IslamShoba Ranganathan
Oct 5, 1997·Biopolymers·S E BlondelleE Pérez-Payá
Feb 1, 1996·Genes, Chromosomes & Cancer·T M HortonD C Wallace
Sep 1, 1996·Journal of Molecular Recognition : JMR·M AleanziA Marcipar
Jul 9, 1999·Journal of Molecular Recognition : JMR·V BöttgerB Micheel
Feb 15, 2001·Proteins·H Naderi-ManeshA A Moosavi Movahedi
Jul 25, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·F MwaleA R Poole
Jan 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H ImamuraH Demura
Jan 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C M Gundberg, M E Clough
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R CernýT Wurtz
Oct 1, 1992·Journal of Cellular Biochemistry·F ColeD S Kohtz
Oct 1, 1994·Journal of Cellular Biochemistry·W G Hoeck, V R Mukku
Oct 1, 1990·Journal of Molecular Recognition : JMR·L G FägerstamH Butt
Aug 14, 2001·Journal of Molecular Recognition : JMR·A B EdmundsonP A Ramsland
Jan 31, 2003·Journal of Molecular Recognition : JMR·Michael Odorico, Jean-Luc Pellequer
Jun 26, 2001·Journal of Medical Virology·Q H WangH Ushijima
Jan 1, 1993·Journal of Medical Virology·L JinJ P Clewley
Dec 1, 1995·Journal of Medical Virology·K Kidd-LjunggrenS Lolekha
Jun 1, 1991·Journal of Neuroscience Research·A NegroL Callegaro
May 11, 1992·Protein Science : a Publication of the Protein Society·A RandolphF R Masiarz
Sep 1, 1994·Protein Science : a Publication of the Protein Society·R LohmannP Wrede
Sep 1, 1995·Protein Science : a Publication of the Protein Society·T P Hopp
Jul 9, 1998·Protein Science : a Publication of the Protein Society·A FrogerC Delamarche
Apr 5, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·Aristomenis MetaxasMaria Liakopoulou-Kyriakide
Jan 14, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Vassiliki A Boumba, Konstantin Seferiadis
Jun 29, 2000·Biochemical and Biophysical Research Communications·M HernándezA Melchor
Mar 1, 1987·Plant Molecular Biology·R P WiseB G Gengenbach

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.