Prediction of renal allograft rejection by urinary protein analysis using ProteinChip Arrays (surface-enhanced laser desorption/ionization time-of-flight mass spectrometry)

Urology
Olaf ReicheltKerstin Junker

Abstract

To develop a noninvasive method for the detection of renal transplant rejection using ProteinChip Arrays (surface-enhanced laser desorption/ionization time-of-flight mass spectrometry). A total of 23 urine samples were collected from 13 patients showing biopsy-proven renal allograft rejection and from 10 patients without histologic signs of rejection. All 23 patients had clinical symptoms and signs of acute allograft rejection and underwent renal biopsy. Samples were centrifuged, and supernatants were directly spotted onto the ProteinChip arrays with different chromatographic surfaces. The obtained spectra in a range from 2 to 200 kDa were subjected to bioinformatic analysis using the method of Fuzzy c-means, followed by the establishment of rule bases and evaluation using the relevance index according to Kiendl. Several protein peaks were identified allowing differentiation between rejection and no rejection. Using two different ProteinChip surfaces, we found two biomarkers at 25.71 kDa and 28.13 kDa that gave a diagnostic sensitivity of 90% and 93% and a specificity of 80% (SAX2) and 85% (CM10), respectively. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry appears to be a promising new diagnostic...Continue Reading

References

Apr 1, 1991·Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology·T AkiyamaT Kurita
Feb 1, 1996·Kidney International·Z A MassyB L Kasiske
Aug 14, 1998·Nature Biotechnology·K Schütze, G Lahr
May 16, 2002·Transplantation Proceedings·Agnieszka PerkowskaM Lao
May 2, 2003·Annals of Surgery·William ClarkeErnesto P Molmenti
Nov 20, 2003·Mass Spectrometry Reviews·Ning TangScot R Weinberger
Dec 12, 2003·The New England Journal of Medicine·Robert B Colvin
Dec 25, 2003·Journal of the American Society of Nephrology : JASN·Stefan SchaubPeter Nickerson

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Citations

Aug 1, 2009·Current Opinion in Organ Transplantation·Minnie M Sarwal
Aug 24, 2012·Current Opinion in Nephrology and Hypertension·Sacha A De SerresAdeera Levin
May 1, 2007·Proteomics. Clinical Applications·Oliver DriemelFerdinand von Eggeling
Sep 4, 2008·Pediatric Transplantation·Tara K Sigdel, Minnie M Sarwal
May 1, 2008·Proteomics. Clinical Applications·Wei GuYi-Ding Chen
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Apr 27, 2011·Proteomics. Clinical Applications·Tara K SigdelMinnie M Sarwal
Jun 15, 2010·Proteomics. Clinical Applications·Tara K SigdelMinnie M Sarwal
Sep 13, 2011·Seminars in Immunology·Minnie M SarwalDaniel R Salomon
Jul 10, 2016·Life Sciences·Juliana D PerezDulce E Casarini
Mar 18, 2010·The Journal of International Medical Research·H YangS Zheng
Mar 25, 2016·World Journal of Transplantation·Wilfried GwinnerDavid Marx
Nov 7, 2017·Expert Review of Proteomics·Arjun Chakraborty, Minnie Sarwal
Feb 4, 2012·Journal of the American Society of Nephrology : JASN·Tara K SigdelMinnie M Sarwal
Jul 16, 2008·Journal of Proteome Research·Ji-Na WangYin-Long Guo

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