Prediction of the combined effects of multiple estrogenic chemicals on MCF-7 human breast cancer cells and a preliminary molecular exploration of the estrogenic proliferative effects and related gene expression.

Ecotoxicology and Environmental Safety
Shengwu YuanZijian Wang

Abstract

The environmental risks of environmental estrogens (EEs) are often assessed via the same mode of action in the concentration addition (CA) model, neglecting the complex combined mechanisms at the genetic level. In this study, the cell proliferation effects of estrone, 17α-ethinylestradiol, 17β-estradiol, estriol, diethylstilbestrol, estradiol valerate, bisphenol A, 4-tert-octylphenol and 4-nonylphenol were determined individually using the CCK-8 method, and the proliferation effects of a multicomponent mixture of estrogenic chemicals mixed at equipotent concentrations using a fixed-ratio design were studied using estrogen-sensitive MCF-7 cells. Furthermore, transcription factors related to cell proliferation were analyzed using RT-PCR assays to explore the potential molecular mechanisms related to the estrogenic proliferative effects. The results showed that the estrogenic chemicals act together in an additive mode, and the combined proliferative effects could be predicted more accurately by the response addition model than the CA model with regard to their adverse outcomes. Furthermore, different signaling pathways were involved depending on the different mixtures. The RT-PCR analyses showed that different estrogens have disti...Continue Reading

Citations

Jan 9, 2019·Environmental Science and Pollution Research International·Fengsong ZhangLinsheng Yang
Aug 25, 2020·Reviews on Environmental Health·William H GoodsonDavid O Carpenter
Dec 18, 2020·Frontiers in Nutrition·Barbara J StillwaterOrnella I Selmin
Feb 20, 2020·Ecotoxicology and Environmental Safety·Qing HuGuang-Bo Ge

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